Development of an indirect ELISA with epitope on nonstructural protein of Muscovy duck parvovirus for differentiating between infected and vaccinated Muscovy ducks

被引：4
作者：
Yan, B. [1 ,2 ]
Ma, J-Z. [1 ]
Yu, T-F. [2 ]
Shao, S-L. [2 ]
Li, M. [3 ]
Fan, X-D. [4 ]
机构：
[1] Northeast Forestry Univ, Coll Wildlife Resources, Harbin 150040, Peoples R China
[2] Qiqihar Univ, Coll Life Sci & Agr Forestry, Qiqihar 161006, Peoples R China
[3] Qiqihar Univ, Coll Comp & Control Engn, Qiqihar 161006, Peoples R China
[4] Heilongjiang Inst Vet Med Sci, Qiqihar, Peoples R China
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2014年 / 59卷 / 06期
基金：
黑龙江省自然科学基金;
关键词：
differential diagnosis; ELISA; epitope; Muscovy duck parvovirus; nonstructural protein; GOOSE PARVOVIRUS; ANTIBODIES; ASSAY;
D O I：
10.1111/lam.12323
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) based on epitope AA503-509 (RANEPKE), which is on nonstructural protein of Muscovy duck parvovirus (MDPV). Sera (100) from negative and vaccinated Muscovy ducks were compared with infected sera (240) to establish the cut-off value of this i-ELISA. There was a significant difference between the positive and negative populations (P<005). The adoption of this positive-negative threshold value for this i-ELISA assay resulted in specificity of 980%. This i-ELISA could be used as a diagnostic tool for differentiating infected Muscovy ducks from Muscovy ducks vaccinated with inactivated virus.
引用
收藏
页码：631 / 635
页数：5
相关论文
共 12 条
  • [1] The development of an indirect ELISA for the detection of antibodies to goose parvovirus in blood serum
    Fan, J. -H.
    Zuo, Y. -Z.
    Yang, Z.
    Pei, L. -H.
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 2013, 57 (01) : 26 - 32
  • [2] Development of an enzyme-linked immunosorbent assay for Bartonella henselae infection detection
    Ferrara, F.
    Di Niro, R.
    D'Angelo, S.
    Busetti, M.
    Marzari, R.
    Not, T.
    Sblattero, D.
    [J]. LETTERS IN APPLIED MICROBIOLOGY, 2014, 59 (03) : 253 - 262
  • [3] Molecular detection of Muscovy duck parvovirus by loop-mediated isothermal amplification assay
    Ji, J.
    Xie, Q. M.
    Chen, C. Y.
    Bai, S. W.
    Zou, L. S.
    Zuo, K. J.
    Cao, Y. C.
    Xue, C. Y.
    Ma, J. Y.
    Bi, Y. Z.
    [J]. POULTRY SCIENCE, 2010, 89 (03) : 477 - 483
  • [4] LesGall-Recule G., 1994, ARCH VIROL, V139, P121
  • [5] LesGall-Recule G., 1996, J GEN VIROL, V77, P2159
  • [6] Immunologic cross-reactivity between Muscovy duck parvovirus and goose parvovirus on the basis of epitope prediction
    Li, Ming
    Yu, Tian-fei
    [J]. BRAZILIAN JOURNAL OF MICROBIOLOGY, 2013, 44 (02) : 519 - 521
  • [7] Isolation and molecular characterization of a new Muscovy duck parvovirus from Muscovy ducks in the USA
    Poonia, Bhawna
    Dunn, Patricia A.
    Lu, Huaguang
    Jarosinski, Keith W.
    Schat, Karel A.
    [J]. AVIAN PATHOLOGY, 2006, 35 (06) : 435 - U4
  • [8] Localization of infection-related epitopes on the non-structural protein 3ABC of foot-and-mouth disease virus and the application of tandem epitopes
    Sun, T
    Lu, P
    Wang, X
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2004, 119 (02) : 79 - 86
  • [9] Expression of capsid proteins and non-structural proteins of waterfowl parvoviruses in Escherichia coli and their use in serological assays
    Wang, CY
    Shieh, HK
    Shien, JH
    Ko, CY
    Chang, PC
    [J]. AVIAN PATHOLOGY, 2005, 34 (05) : 376 - 382
  • [10] Evidence of Muscovy duck parvovirus in Muscovy ducklings in California
    Woolcock, PR
    Jestin, V
    Shivaprasad, HL
    Zwingelstein, F
    Arnauld, C
    McFarland, MD
    Pedersen, JC
    Senne, DA
    [J]. VETERINARY RECORD, 2000, 146 (03) : 68 - 72
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