Cell detachment and label-free cell sorting using modulated surface acoustic waves (SAWs) in droplet-based microfluidics

被引：36

作者：

Bussonniere, Adrien ^{[1
,2
]}

Miron, Yannick ^{[3
]}

Baudoin, Michael ^{[1
,2
]}

Matar, Olivier Bou ^{[1
,2
]}

Grandbois, Michel ^{[3
]}

Charette, Paul ^{[4
,5
]}

Renaudin, Alan ^{[4
,5
]}

机构：

[1] Univ Lille 1, UMR CNRS 8520, Inst Elect Microelect & Nanotechnol, LIA LEMAC LICS, F-59652 Villeneuve Dascq, France

[2] EC Lille, F-59652 Villeneuve Dascq, France

[3] Univ Sherbrooke, Fac Med & Sci Sante, Dept Pharmacol, Sherbrooke, PQ J1H 5N4, Canada

[4] Univ Sherbrooke, CNRS UMI 3463, Lab Nanotechnol Nanosyst LN2, Sherbrooke, PQ J1K OA5, Canada

[5] Univ Sherbrooke, CNRS UMI 3463, Lab Nanotechnol Nanosyst LN2, Sherbrooke, PQ J1K OA5, Canada

来源：

LAB ON A CHIP | 2014年 / 14卷 / 18期

基金：

加拿大自然科学与工程研究理事会;

关键词：

BREAST-CANCER CELLS; SEPARATION; PARTICLE; BLOOD; FLOW; MANIPULATION; ENRICHMENT; DRIVEN; DEVICE;

D O I：

10.1039/c4lc00625a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a droplet-based surface acoustic wave (SAW) system designed to viably detach biological cells from a surface and sort cell types based on differences in adhesion strength (adhesion contrast) without the need to label cells with molecular markers. The system uses modulated SAW to generate pulsatile flows in the droplets and efficiently detach the cells, thereby minimizing the SAW excitation power and exposure time. As a proof of principle, the system shows efficient sorting of HEK 293 from A7r5 cells based on adhesion contrast. Results are obtained in minutes with sorting purity and efficiency reaching 97% and 95%, respectively.

引用

页码：3556 / 3563

页数：8

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