On-chip magnetophoretic isolation of CD4+T cells from blood

被引:24
作者
Darabi, Jeff [1 ]
Guo, Chuan [1 ]
机构
[1] So Illinois Univ, Dept Mech Engn, Edwardsville, IL 62026 USA
关键词
HUMAN PERIPHERAL-BLOOD; SEPARATION; MACS; FLOW; CYTOMETRY; CAPTURE;
D O I
10.1063/1.4821628
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This paper presents the design, fabrication, and testing of a magnetophoretic bioseparation chip for the rapid isolation and concentration of CD4 + T cells from the peripheral blood. In a departure from conventional magnetic separation techniques, this microfluidic-based bioseperation device has several unique features, including locally engineered magnetic field gradients and a continuous flow with a buffer switching scheme to improve the performance of the separation process. Additionally, the chip is capable of processing significantly smaller sample volumes than conventional methods and sample losses are eliminated due to decreased handling. Furthermore, the possibility of sample-to-sample contamination is reduced with the disposable format. The overall dimensions of the device were 22 mm by 60 mm by 1 mm, approximately the size of a standard microscope slide. The results indicate a cell purity of greater than 95% at a sample flow rate of 50 ml/h and a cell recovery of 81% at a sample flow rate of 10 ml/h. The cell purity was found to increase with increasing the sample flow rate. However, the cell recovery decreases with an increase in the flow rate. A parametric study was also performed to investigate the effects of channel height, substrate thickness, magnetic bead size, and number of beads per cell on the cell separation performance. (C) 2013 AIP Publishing LLC.
引用
收藏
页数:14
相关论文
共 34 条
[1]  
[Anonymous], 1999, CLASSICAL ELECTRODYN
[2]  
Bartholomeusz DA, 2005, J MICROELECTROMECH S, V14, P1364, DOI 10.1109/JMEMS.2005.859087
[3]   Microfluidics for cell separation [J].
Bhagat, Ali Asgar S. ;
Bow, Hansen ;
Hou, Han Wei ;
Tan, Swee Jin ;
Han, Jongyoon ;
Lim, Chwee Teck .
MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING, 2010, 48 (10) :999-1014
[4]  
Bleaney B.I., 1989, ELECT MAGNETISM, V3rd
[5]  
Brosterhus H, 1999, EUR J IMMUNOL, V29, P4053, DOI 10.1002/(SICI)1521-4141(199912)29:12<4053::AID-IMMU4053>3.3.CO
[6]  
2-3
[7]   Characterization of a microfluidic magnetic bead separator for high-throughput applications [J].
Bu, Minqiang ;
Christensen, Troels B. ;
Smistrup, Kristian ;
Wolff, Anders ;
Hansen, Mikkel F. .
SENSORS AND ACTUATORS A-PHYSICAL, 2008, 145 :430-436
[8]   Microstripes for transport and separation of magnetic particles [J].
Donolato, Marco ;
Dalslet, Bjarke Thomas ;
Hansen, Mikkel Fougt .
BIOMICROFLUIDICS, 2012, 6 (02)
[9]   Isolation of prostate cancer cell subpopulations of functional interest by use of an on-chip magnetic bead-based cell separator [J].
Estes, Matthew D. ;
Ouyang, Bin ;
Ho, Shuk-mei ;
Ahn, Chong H. .
JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2009, 19 (09)
[10]   Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems [J].
Furdui, VI ;
Harrison, DJ .
LAB ON A CHIP, 2004, 4 (06) :614-618