Polyphasic characterization of the thermotolerant cyanobacterium Desertifilum sp strain IPPAS B-1220

被引：18

作者：

Sinetova, Maria A. ^{[1
]}

Bolatkhan, Kenzhegul ^{[2
]}

Sidorov, Roman A. ^{[1
]}

Mironov, Kirill S. ^{[1
]}

Skrypnik, Alexandra N. ^{[1
]}

Kupriyanova, Elena V. ^{[1
]}

Zayadan, Bolatkhan K. ^{[2
]}

Shumskaya, Maria ^{[3
]}

Los, Dmitry A. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Plant Physiol, Botanicheskaya St 35, Moscow 127276, Russia

[2] Al Farabi Kazakh Natl Univ, Fac Biol & Biotechnol, Dept Biotechnol, Alma Ata, Kazakhstan

[3] Kean Univ, Sch Nat Sci, Dept Biol, 1000 Morris Ave, Union, NJ 07083 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2017年 / 364卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

Desertifilum; molecular phylogeny; polyphasic approach; ultrastructure; fatty acids; fatty acid desaturases; FATTY-ACID-COMPOSITION; BLUE-GREEN-ALGAE; BIOSYNTHESIS; DESATURATION; DOMAINS; GENES; MODES; DNA;

D O I：

10.1093/femsle/fnx027

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A cyanobacterial strain from Lake Shar-Nuur, a freshwater lake in Mongolia, was isolated and characterized by a polyphasic approach. According to the 16S ribosomal RNA gene sequence, this strain (IPPAS B-1220) belongs to a newly described genus Desertifilum. In general, strains of Desertifilum maintain their genetic stability, as seen from the analysis of the 16S rRNA gene and 16S-23S rRNA internal transcribed spacer sequences from strains collected at distant locations. The newly discovered strain is characterized by an unusual fatty acid composition (16: 1 Delta(7) and 16: 2 Delta(7,10)). Analysis of its draft genomic sequence reveals the presence of six genes for the acyl-lipid desaturases: two Delta 9-desaturases, desC1 and desC2; two Delta 12-desaturases, desA1 and desA2; one desaturase of unknown specificity, desX; and one gene for the bacillary-type desaturase, desG, which supposedly encodes an.9-desaturase. A scheme for a fatty acid desaturation pathway that describes the biosynthesis of 16: 1 Delta(7) and 16: 2 Delta(7,10) fatty acids in Desertifilum is proposed.

引用

页数：10

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