Mating flight causes genome-wide transcriptional changes in sexually mature honeybee queens

被引：8

作者：

Wu, Xiaobo ^{[1
]}

Wang, Zilong ^{[1
]}

Zhang, Fei ^{[1
]}

Shi, Yuanyuan ^{[1
]}

Zeng, Zhijiang ^{[1
]}

机构：

[1] Jiangxi Agr Univ, Honeybee Res Inst, Nanchang 330045, Jiangxi, Peoples R China

来源：

JOURNAL OF ASIA-PACIFIC ENTOMOLOGY | 2014年 / 17卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Apis mellifera; Mating flight; Digital gene expression; miRNA; Expression difference; FEMALE REPRODUCTIVE-TRACT; GENE-EXPRESSION; APIS-MELLIFERA; DROSOPHILA-MELANOGASTER; MICRORNAS; TRANSLATION; BIOGENESIS; TISSUES; NUMBER; BEES;

D O I：

10.1016/j.aspen.2013.10.001

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

In this study, we analyzed the gene and miRNA expression differences between the courted virgin queen (CVQ) and non-courted virgin queen (NCVQ) of Apis mellifera using a high-throughput sequencing method. Through Digital Gene Expression (DGE) sequencing, 452 genes were differentially expressed, out of which, 90 genes were up-regulated and 362 genes were down-regulated in CVQ compared with NCVQ. Through small RNA sequencing, 27 miRNAs showed significant expression difference between these two samples. Moreover, 9 of the differentially expressed genes are the targets of the 11 differentially expressed miRNAs. Besides, 47 novel miRNA candidates were predicted in these two samples. Our results provided valuable information for understanding the molecular mechanism of the transition to functional queens. Crown Copyright (C) 2013 Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. All rights reserved.

引用

页码：37 / 43

页数：7

共 29 条

[1]

ADAMS J, 1977, GENETICS, V86, P583

[2]

Allen E, 2005, CELL, V121, P207, DOI 10.1016/j.cell.2005.04.004

[3] The significance of digital gene expression profiles [J].

Audic, S ;

Claverie, JM .

GENOME RESEARCH, 1997, 7 (10) :986-995

[4] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[5] microRNA miR-275 is indispensable for blood digestion and egg development in the mosquito Aedes aegypti [J].

Bryant, Bart ;

Macdonald, Warren ;

Raikhel, Alexander S. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (52) :22391-22398

[6] RNA sequence analysis defines Dicer's role in mouse embryonic stem cells [J].

Calabrese, J. Mauro ;

Seila, Amy C. ;

Yeo, Gene W. ;

Sharp, Phillip A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (46) :18097-18102

[7] MicroRNA in the ovary and female reproductive tract [J].

Carletti, M. Z. ;

Christenson, L. K. .

JOURNAL OF ANIMAL SCIENCE, 2009, 87 (14) :E29-E38

[8] Next-generation small RNA sequencing for microRNAs profiling in the honey bee Apis mellifera [J].

Chen, X. ;

Yu, X. ;

Cai, Y. ;

Zheng, H. ;

Yu, D. ;

Liu, G. ;

Zhou, Q. ;

Hu, S. ;

Hu, F. .

INSECT MOLECULAR BIOLOGY, 2010, 19 (06) :799-805

[9] The microRNA miR-124 controls gene expression in the sensory nervous system of Caenorhabditis elegans [J].

Clark, Alejandra M. ;

Goldstein, Leonard D. ;

Tevlin, Maya ;

Tavare, Simon ;

Shaham, Shai ;

Miska, Eric A. .

NUCLEIC ACIDS RESEARCH, 2010, 38 (11) :3780-3793

[10] Dynamic, mating-induced gene expression changes in female head and brain tissues of Drosophila melanogaster [J].

Dalton, Justin E. ;

Kacheria, Tanvi S. ;

Knott, Simon R. V. ;

Lebo, Matthew S. ;

Nishitani, Allison ;

Sanders, Laura E. ;

Stirling, Emma J. ;

Winbush, Ari ;

Arbeitman, Michelle N. .

BMC GENOMICS, 2010, 11

← 1 2 3 →