Inflight Polymerase Chain Reaction of samples with drones

被引：3
作者：
Abid, Hassan Ali [1 ]
Minifie, Tristan [1 ]
Ong, Jian Wern [1 ]
Lin, Eric Shen [1 ]
Song, Zhixiong [1 ]
Liew, Oi Wah [2 ]
Ng, Tuck Wah [1 ]
机构：
[1] Monash Univ, Lab Opt & Appl Mech, Dept Mech & Aerosp Engn, Clayton, Vic 3800, Australia
[2] Natl Univ Singapore, Natl Univ Hlth Syst, Ctr Translat Med, Cardiovasc Res Inst,Yong Loo Lin Sch Med, 14 Med Dr, Singapore 117599, Singapore
来源：
ANALYTICAL BIOCHEMISTRY | 2021年 / 616卷
关键词：
Drone; UAV; Quadcopter; PCR; Transport; TIME; PCR;
D O I：
10.1016/j.ab.2020.114098
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A system devised to conduct Polymerase Chain Reaction (PCR) in-flight on drones that uses the spatial displacement of capillary tubes on thermal blocks kept at 94 degrees C, 58 degrees C and 72 degrees C corresponding to cycling temperatures for denaturation, annealing and extension is demonstrated here. The use of acetal as the thermal block material reduced heat loss and the input power (within 18.5 W) needed to maintain the required temperatures. Tests showed that concentrations of samples down to 1.16 x 10 (6) DNA copies/mu L could be significantly and consistently detected above the background emission of the fluorescence signal intensity.
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页数：3
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