Differentiation of fish species in Taiwan Strait by PCR-RFLP and lab-on-a-chip system

被引：21

作者：

Chen, Shuangya ^{[1
]}

Zhang, Yongxiang ^{[2
]}

Li, Hong ^{[3
]}

Wang, Jiahe ^{[1
]}

Chen, Weiling ^{[1
]}

Zhou, Yu ^{[1
]}

Zhou, Shan ^{[4
]}

机构：

[1] Xiamen Entry Exit Inspect & Quarantine Technol Ct, Xiamen 361026, Peoples R China

[2] Xinglin Entry Exit Inspect & Quarantine Bur, Xiamen 361022, Peoples R China

[3] China Natl Accreditat Serv Conform Assessment, Beijing 100088, Peoples R China

[4] Agilent Technol Co Ltd, Beijing 100102, Peoples R China

来源：

FOOD CONTROL | 2014年 / 44卷

关键词：

Fish species identification; Taiwan Strait; PCR-RFLP; Lab-on-a-chip; MULTIPLEX PCR; PUFFER FISH; IDENTIFICATION;

D O I：

10.1016/j.foodcont.2014.03.019

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and lab-on-a-chip system were used to identify 62 commercial fish species in Taiwan Strait The fish species include 10 groupers, 12 bream species, 9 Sciaenidae species, 5 puffer species and 26 other fish species. A fragment of 464 bp length of mitochondrial cytoehrome b gene was amplified by PCR and the products were digested with restriction enzymes DdeI, HaeIII and NiaIII, individually. The fragments generated after digestion were further resolved on the DNA chip. The results demonstrated that PCR-RFLP analysis and lab-on-a-chip system provided a fast, easy, automated and reliable analysis approach and it will be useful for the control of the adulteration of food with fish tissue content in Taiwan Strait. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：26 / 34

页数：9

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