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Cloning and expression of synthetic genes encoding native, hybrid- and bacteriocin-derived chimeras from mature class IIa bacteriocins, by Pichia pastoris (syn. Komagataella spp.)
被引:21
作者:

Arbulu, Sara
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机构:
UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain
Univ Coll Cork, APC Microbiome Ireland, Cork, Ireland
TEAGASC, Food Res Ctr, Fermoy, Cork, Ireland UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain

Jimenez, Juan J.
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机构:
UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain
ACD Pharmaceut AS, Leknes, Norway UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain

Gutiez, Loreto
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机构:
UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain
CESAG Univ Pontificia Comillas, Palma De Mallorca, Spain UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain

Feito, Javier
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UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain

Cintas, Luis M.
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UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain

Herranz, Carmen
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机构:
UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain

Hernandez, Pablo E.
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机构:
UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain
机构:
[1] UCM, Secc Dept Nutr & Ciencia Alimentos, Nutr Bromatol Higene & Seguridad Alimentaria, Fac Vet, Ave Puerta Hierro S-N, Madrid 28040, Spain
[2] Univ Coll Cork, APC Microbiome Ireland, Cork, Ireland
[3] TEAGASC, Food Res Ctr, Fermoy, Cork, Ireland
[4] ACD Pharmaceut AS, Leknes, Norway
[5] CESAG Univ Pontificia Comillas, Palma De Mallorca, Spain
关键词:
Synthetic genes;
Native bacteriocins;
Hybrid- and bacteriocin-derived chimeras;
Heterologous bacteriocin production;
Pichia pastoris;
MALDI-TOF MS analysis;
MRM-ESI-LC-MS/MS (QTRAP) analysis;
LACTIC-ACID BACTERIA;
ENTEROCOCCUS-FAECIUM T136;
SEC-DEPENDENT BACTERIOCIN;
FUNCTIONAL EXPRESSION;
ANTIMICROBIAL PEPTIDE;
IMMUNITY;
RECOGNITION;
OPTIMIZATION;
P13;
D O I:
10.1016/j.foodres.2019.01.015
中图分类号:
TS2 [食品工业];
学科分类号:
0832 ;
摘要:
In this work, synthetic genes designed from (a), the native amino acid sequence of the class IIa bacteriocins enterocin HF (EntHF) and enterocin CRL35 (EntCRL35), (b) from hybrid bacteriocins derived from fusion of enterocin A (EntA) to itself and to EntHF and EntCRL35 through a tri-glycine peptide linker, and (c) from bacteriocin-derived chimeras devised from fusion of the N-terminal region of EntA and enterocin P (EntP) to the C-terminal end of EntHF and EntCRL35, were cloned in plasmid pPICZ alpha A for expression by P. pastoris X-33. Synthetic genes encoding EntHF and EntCRL35 were also cloned in plasmid pP-alpha hSUMO3 for expression of the hSUMO3-fused bacteriocins by P. pastoris. Only recombinant P. pastoris expressing the bacteriocin-derived chimeras displayed a direct antimicrobial activity whereas P. pastoris X-33, producer of EntP::EntHF, showed the highest antimicrobial activity in their supernatants and in the multi-step chromatographic purified fractions. The MRM-ESI-LC-MS/MS (QTRAP) analysis of purified fractions from P. pastoris producers of hybrid- and bacteriocin-derived chimeras, permitted detection in the samples of peptides with the expected molecular mass of the bacteriocins produced. The antimicrobial activity of the EntP::EntHF chimera compared to that of the synthetic EntP::EntHF peptide, suggest that the biologically-produced bacteriocin-derived chimera shows a higher specific antimicrobial activity than its synthetic counterpart against different Listeria strains, including L. monocytogenes. More important, the N-terminal region of EntA and EntP seems to drive the production, processing and secretion of hybrid- and bacteriocin-derived chimeras, by P. pastoris X-33.
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页码:888 / 899
页数:12
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[J].
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;
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;
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;
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Cintas, Luis M.
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[7]
Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by Recombinant Pichia pastoris
[J].
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;
Jimenez, Juan J.
;
Gutiez, Loreto
;
Cintas, Luis M.
;
Herranz, Carmen
;
Hernandez, Pablo E.
.
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2015, 2015

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[8]
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[J].
Borrero, Juan
;
Kunze, Gotthard
;
Jimenez, Juan J.
;
Boeer, Erik
;
Gutiez, Loreto
;
Herranz, Carmen
;
Cintas, Luis M.
;
Hernandez, Pablo E.
.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY,
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, Madrid, Spain
[9]
Protein expression vector and secretion signal peptide optimization to drive the production, secretion, and functional expression of the bacteriocin enterocin A in lactic acid bacteria
[J].
Borrero, Juan
;
Jimenez, Juan J.
;
Gutiez, Loreto
;
Herranz, Carmen
;
Cintas, Luis M.
;
Hernandez, Pablo E.
.
JOURNAL OF BIOTECHNOLOGY,
2011, 156 (01)
:76-86

Borrero, Juan
论文数: 0 引用数: 0
h-index: 0
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Jimenez, Juan J.
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Gutiez, Loreto
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Herranz, Carmen
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Cintas, Luis M.
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Hernandez, Pablo E.
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain
[10]
Characterization of Garvicin ML, a Novel Circular Bacteriocin Produced by Lactococcus garvieae DCC43, Isolated from Mallard Ducks (Anas platyrhynchos)
[J].
Borrero, Juan
;
Brede, Dag A.
;
Skaugen, Morten
;
Diep, Dzung B.
;
Herranz, Carmen
;
Nes, Ingolf F.
;
Cintas, Luis M.
;
Hernandez, Pablo E.
.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY,
2011, 77 (01)
:369-373

Borrero, Juan
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Brede, Dag A.
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Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, Lab Microbial Gene Technol, N-1432 As, Norway Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Skaugen, Morten
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Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, Lab Microbial Gene Technol, N-1432 As, Norway Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Diep, Dzung B.
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Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, Lab Microbial Gene Technol, N-1432 As, Norway Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Herranz, Carmen
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Nes, Ingolf F.
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Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, Lab Microbial Gene Technol, N-1432 As, Norway Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Cintas, Luis M.
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain

Hernandez, Pablo E.
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Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain