Simultaneous production of fatty acid methyl esters and diglycerides by four recombinant Candida rugosa lipase's isozymes

被引：18

作者：

Chang, Shu-Wei ^{[2
]}

Huang, Myron ^{[3
]}

Hsieh, Yu-Hsun ^{[4
]}

Luo, Ying-Ting ^{[5
]}

Wu, Tsung-Ta ^{[6
]}

Tsai, Chia-Wen ^{[7
]}

Chen, Chin-Shuh ^{[3
]}

Shaw, Jei-Fu ^{[1
,3
,4
,8
,9
]}

机构：

[1] I Shou Univ, Dept Biol Sci & Technol, Kaohsiung 84001, Taiwan

[2] Dayeh Univ, Dept Med Bot & Hlth Applicat, Changhua 515, Taiwan

[3] Natl Chung Hsing Univ, Dept Food Sci & Biotechnol, Taichung 402, Taiwan

[4] Natl Chung Hsing Univ, Dept Life Sci, Taichung 402, Taiwan

[5] Dayeh Univ, Dept Bioind Technol, Changhua 515, Taiwan

[6] Natl Chung Hsing Univ, Dept Chem Engn, Taichung 402, Taiwan

[7] China Med Univ, Dept Nutr, Taichung 404, Taiwan

[8] Acad Sinica, Agr Biotechnol Res Ctr, Taipei 11529, Taiwan

[9] Natl Chung Hsing Univ, Agr Biotechnol Ctr, Taichung 402, Taiwan

来源：

FOOD CHEMISTRY | 2014年 / 155卷

关键词：

Candida rugosa lipase; Diacylglycerol; Emulsifier; Esterification; Fatty acid methyl ester; Isozymes; ACTIVATED BLEACHING EARTH; BIODIESEL FUEL PRODUCTION; PICHIA-PASTORIS; BIOCHEMICAL-CHARACTERIZATION; MULTIPLE MUTAGENESIS; CATALYZED PRODUCTION; CODON OPTIMIZATION; IMMOBILIZED LIPASE; LIP2; LIPASE; OIL;

D O I：

10.1016/j.foodchem.2014.01.035

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, the catalytic efficiency of four recombinant CRL (Candida rugosa lipase) isozymes (LIP1-LIP4) towards the production of fatty acid methyl ester (FAME) was compared and evaluated as an alternative green method for industrial applications. The results indicated that the recombinant C. rugosa LIP1 enzyme exhibited the highest catalytic efficiency for FAME production compared to the recombinant C rugosa LIP2-LIP4 enzymes. The optimal conditions were as follows: pH 7.0, methanol/soybean oil molar ratio: 3/1, enzyme amount: 2 U (1.6 mu L) reaction temperature: 20 degrees C, 22 h of reaction time, and 3 times of methanol addition (1 mol/6 h), and resulted in 61.5 +/- 1.5 wt.% of FAME conversion. The reaction product contained also 10 wt.% of DAG with a ratio of 1,3-DAG to 1,2-DAG of approximately 4:6, and can be potentially used in industrial applications as a food emulsifier. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：140 / 145

页数：6

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