Microfluidics for the analysis of behavior, nerve regeneration, and neural cell biology in C. elegans

被引:92
作者
Ben-Yakar, Adela [2 ]
Chronis, Nikos [1 ,3 ]
Lu, Hang [4 ]
机构
[1] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA
[2] Univ Texas Austin, Dept Mech Engn, Austin, TX 78705 USA
[3] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[4] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA
基金
美国国家科学基金会;
关键词
OLFACTORY BEHAVIOR; NEURONS; IMMOBILIZATION; CIRCUIT; SYSTEM;
D O I
10.1016/j.conb.2009.10.010
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The nematode Caenorhabditis elegans is a widely adopted model organism for studying various neurobiological processes at the molecular and cellular level in vivo. With a small, flexible, and continuously moving body, the manipulation of C. elegans becomes a challenging task. In this review, we highlight recent advances in microfluidic technologies for the manipulation of C. elegans. These new family of microfluidic chips are capable of handling single or populations of worms in a high-throughput fashion and accurately controlling their microenvironment. So far, they have been successfully used to study neural circuits and behavior, to perform large-scale phetotyping and morphology-based screens as well as to understand axon regeneration after injury. We envision that microfluidic chips can further be used to study different aspects of the C. elegans nervous system, extending from fundamental understanding of behavioral dynamics to more complicated biological processes such as neural aging and learning and memory.
引用
收藏
页码:561 / 567
页数:7
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