Quantification of Antioxidant Phenolic Compounds in a New Chrysanthemum Cultivar by High-Performance Liquid Chromatography with Diode Array Detection and Electrospray Ionization Mass Spectrometry

被引：22

作者：

Han, Ah-Reum ^{[1
]}

Kim, Hyo Young ^{[1
]}

So, Yangkang ^{[1
]}

Nam, Bomi ^{[1
,2
]}

Lee, Ik-Soo ^{[2
]}

Nam, Joo-Won ^{[3
]}

Jo, Yeong Deuk ^{[1
]}

Kim, Sang Hoon ^{[1
]}

Kim, Jin-Baek ^{[1
]}

Kang, Si-Yong ^{[1
]}

Jin, Chang Hyun ^{[1
]}

机构：

[1] Korea Atom Energy Res Inst, Adv Radiat Technol Inst, Jeongeup Si 56212, Jeollabuk Do, South Korea

[2] Chonnam Natl Univ, Coll Pharm, Gwangju 11686, South Korea

[3] Yeungnam Univ, Coll Pharm, Gyongsan 38541, Gyeongsangbuk D, South Korea

来源：

INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY | 2017年 / 2017卷

基金：

新加坡国家研究基金会;

关键词：

FLAVONOIDS; IDENTIFICATION; MORIFOLIUM; ACIDS;

D O I：

10.1155/2017/1254721

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The flowers of Chrysanthemum morifolium Ramat. have been used as an herbal tea and in traditional medicine, and the plant has been developed to produce horticultural cultivars of various colors and shapes. In this study, a new chrysanthemum cultivar with dark purple petals (C. morifolium cv. ARTI-Dark Chocolate; ADC) was developed by radiation-induced mutation breeding of its original cultivar with purple striped white petals (C. morifolium cv. NobleWine, NW). The phenolic profile and antioxidant property of ADC were investigated and compared with NW and the commercially available medicinal herb, C. morifolium with yellow petals (CM), in order to find a scientific support to produce a new source of natural antioxidant. Flavonoid and phenolic acid profiles of the ethanol extracts of the three flowers were analyzed by high-performance liquid chromatography-diode array detector-electrospray ionization mass spectrometry (HPLC-DAD-ESIMS), while antioxidant properties were evaluated using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. Among the tested flowers, ADC possessed the strongest antioxidant capacity and the highest phenolic contents. Flavonoids (acacetin, apigenin, luteolin, acacetin-7-O-beta-glucoside,apigenin-7-O-beta-glucoside, luteolin-7-O-beta-glucoside, and linarin) and phenolic acids (chlorogenic acid and mixture of 1,4-, 1,5-, and 3,5-dicaffeoylquinic acids) were identified and quantified.

引用

页数：8

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