The Effect of Tetramethylpyrazine on Hydrogen Peroxide-Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells

被引:51
作者
Li, Wen-Ming [1 ]
Liu, Hong-Tao [1 ,2 ]
Li, Xiu-Ying [1 ]
Wu, Jian-Yong [1 ]
Xu, Gang [1 ]
Teng, Yong-Zhen [1 ]
Ding, Song-Tao [1 ]
Yu, Chao [1 ]
机构
[1] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China
[2] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
关键词
NITRIC-OXIDE SYNTHASE; PC12; CELLS; IN-VITRO; H2O2-INDUCED APOPTOSIS; ANTIOXIDANT ACTIVITIES; EXPRESSION; INJURY; ACID; PROLIFERATION; GENERATION;
D O I
10.1111/j.1742-7843.2009.00470.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Tetramethylpyrazine has been widely used in traditional Chinese medicine to treat cardiovascular diseases such as atherosclerosis and hypertension. The underlying mechanism of cardioprotective effects, however, remains to be elucidated. Here, using human umbilical vein endothelial cells (HUVECs), we have assessed the protective effect of tetramethylpyrazine on H2O2-induced oxidative damage. After pre-incubation with tetramethylpyrazine (50, 100 and 150 mu g/ml) for 24 hr., viability loss in H2O2-induced HUVECs (76.5% of the control level, p < 0.05, at 400 mu M of H2O2 for 12 hr.) was restored in a concentration-dependent manner, and the maximal recovery (88.7% of the control level, p < 0.05) was achieved with tetramethylpyrazine at 150 mu g/ml. The production of reactive oxygen species was suppressed by measuring fluorescent intensity of 2',7'-dichorofluorescein (83.1% of the H2O2-treated group, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Tetramethylpyrazine also increased activities of superoxide dismutase and glutathione peroxidase (144.1% and 118.3% of the H2O2-treated group, respectively, p < 0.05 at 150 mu g/ml of tetramethylpyrazine). In addition, tetramethylpyrazine reduced levels of malonaldehyde, intracellular nitric oxide and nitric oxide synthase (83.8%. 91.2% and 78.7% of the H2O2-treated group, respectively, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Furthermore, pre-incubation of tetramethylpyrazine with HUVECs for 24 hr. resulted in reduction of apoptosis and removal of cell cycle arrest in the S phase (56.6% and 59.7% of the H2O2-treated group, respectively, p < 0.01, at 150 mu g/ml of tetramethylpyrazine). Altogether, these results suggest that tetramethylpyrazine has it protective effect oil H2O2-induced oxidative damage in HUVECs due to its antioxidant and antiapoptotic properties.
引用
收藏
页码:45 / 52
页数:8
相关论文
共 50 条
  • [41] Achyranthes bidentata Polypeptide Protects Schwann Cells From Apoptosis in Hydrogen Peroxide-Induced Oxidative Stress
    Li, Meiyuan
    Zhu, Ye
    Peng, Wenqiang
    Wang, Hongkui
    Yuan, Ying
    Gu, Xiaosong
    FRONTIERS IN NEUROSCIENCE, 2018, 12
  • [42] Pomegranate (Punica granatum) phenolics ameliorate hydrogen peroxide-induced oxidative stress and cytotoxicity in human keratinocytes
    Liu, Chang
    Guo, Hao
    DaSilva, Nicholas A.
    Li, Dongli
    Zhang, Kun
    Wan, Yinsheng
    Gao, Xing-Hua
    Chen, Hong-Duo
    Seeram, Navindra P.
    Ma, Hang
    JOURNAL OF FUNCTIONAL FOODS, 2019, 54 : 559 - 567
  • [43] Effects of resveratrol on hydrogen peroxide-induced oxidative stress in embryonic neural stem cells
    Konyalioglu, Sibel
    Armagan, Guliz
    Yalcin, Ayfer
    Atalayin, Cigdem
    Dagci, Taner
    NEURAL REGENERATION RESEARCH, 2013, 8 (06) : 485 - 495
  • [44] Chlorogenic acid attenuates hydrogen peroxide-induced oxidative stress in lens epithelial cells
    Song, Jike
    Guo, Dadong
    Bi, Hongsheng
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2018, 41 (02) : 765 - 772
  • [45] Protection of Bovine Mammary Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Cell Damage by Resveratrol
    Jin, Xiaolu
    Wang, Kai
    Liu, Hongyun
    Hu, Fuliang
    Zhao, Fengqi
    Liu, Jianxin
    OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 2016, 2016
  • [46] Berberine Protects Human Retinal Pigment Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Damage through Activation of AMPK
    Li, Shuai
    Gaur, Uma
    Chong, Cheong-Meng
    Lin, Shaofen
    Fang, Jiankang
    Zeng, Zhiwen
    Wang, Haitao
    Zheng, Wenhua
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (06):
  • [47] Resveratrol attenuates hydrogen peroxide-induced apoptosis, reactive oxygen species generation, and PSGL-1 and VWF activation in human umbilical vein endothelial cells, potentially via MAPK signalling pathways
    Lou, Zhenkai
    Li, Xingguo
    Zhao, Xueling
    Du, Kaili
    Li, Xing
    Wang, Bing
    MOLECULAR MEDICINE REPORTS, 2018, 17 (02) : 2479 - 2487
  • [48] The protective effect of daidzein on high glucose-induced oxidative stress in human umbilical vein endothelial cells
    Park, Mi Hwa
    Ju, Jae-Won
    Kim, Mihyang
    Han, Ji-Sook
    ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 2016, 71 (1-2): : 21 - 28
  • [49] Effect of β-arrestin on damage of human umbilical vein endothelial cell induced by angiotensin II
    Cao, Z. -H.
    Gao, L.
    Jiang, L.
    Zhang, P.
    Ning, H. -Y.
    Zhang, H.
    EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2017, 21 (24) : 5821 - 5826
  • [50] Protective effect of erdosteine metabolite I against hydrogen peroxide-induced oxidative DNA-damage in lung epithelial cells
    Marabini, Laura
    Calo, Rossella
    Braga, Pier Carlo
    ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH, 2011, 61 (12): : 700 - 706