Critical points in the analysis of ciprofloxacin by high-performance liquid chromatography

被引：27

作者：

De Witte, Bavo ^{[1
]}

Dewulf, Jo ^{[1
]}

Demeestere, Kristof ^{[1
]}

De Ruyck, Michael ^{[1
]}

Van Langenhove, Herman ^{[1
]}

机构：

[1] Univ Ghent, Fac Biosci Engn, Res Grp ENVOC, B-9000 Ghent, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1140卷 / 1-2期

关键词：

ciprofloxacin; esterification; pKa; matrix effects;

D O I：

10.1016/j.chroma.2006.11.076

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatographic analysis of the antibiotic compound ciprofloxacin on a C18 column proved to show some critical points. On a C18 column, ciprofloxacin analysis resulted in two peaks in the chromatogram. Effects of stock solution composition, mobile phase composition and type of. stationary phase were investigated to understand the occurrence of both peaks. Esterification of the carboxylic group of ciprofloxacin was shown when methanol was used as stock solution solvent in acidic conditions. Secondly, a ciprofloxacin pK(a) value of 3.64 in water at position N-1 was found which could not fully explain the observation of the two peaks: mobile phase composition and type of stationary phase were also found to be important parameters with respect to the peak area ratio of both peaks. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：126 / 130

页数：5

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