Effects of platelet release products on neutrophilic activity in human whole blood

被引：14

作者：

Wu, B. ^{[2
,5
]}

Liu, G. ^{[3
]}

Yube, K. ^{[4
]}

Ueno, M. ^{[2
]}

Tanaka, S. ^{[2
]}

Onodera, M. ^{[2
]}

Jin, Z. ^{[5
]}

Sakamoto, H. ^{[1
,2
]}

机构：

[1] Kagawa Med Univ, Dept Inflammat Pathol, Miki, Kagawa 7610793, Japan

[2] Kagawa Univ, Fac Med, Dept Inflammat Pathol, Miki, Kagawa 7610793, Japan

[3] Kagawa Univ, Fac Med, Dept Pharmacol, Miki, Kagawa 7610793, Japan

[4] Kagawa Univ, Life Sci Res Ctr, Miki, Kagawa 7610793, Japan

[5] China Med Univ, Affiliated Hosp 2, Dept Gynecol & Obstet, Shenyang 110003, Peoples R China

来源：

INFLAMMATION RESEARCH | 2009年 / 58卷 / 06期

关键词：

Platelet release products; Phagocytosis; Oxidative burst; Macromolecular activators of phagocytosis from platelet; Human whole blood; RESPIRATORY BURST; ACTIN POLYMERIZATION; RESTING PLATELETS; PHAGOCYTOSIS; COMPLEMENT; HEPARIN; ACTIVATORS; RECEPTOR; PATHWAY;

D O I：

10.1007/s00011-009-8230-y

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To investigate the effects of Platelet release products (PRPr) and their phagocytosis activators including ATP, ADP, and macromolecular activators of phagocytosis (MAPPs) on phagocytosis and oxidative burst activity by neutrophils in human whole blood. A whole blood-based flow cytometric assay was used to assess neutrophilic activity. Escherichia coli were used as the target organisms. Phagocytosis and oxidative burst by neutrophils were markedly increased after treatment with PRPr or MAPPs + ATP + ADP. The phagocytosis activation was more prominent in the first minute, and displayed a maximum enhancement of 4-fold. The maximum augmentation of oxidative burst was 5-fold which occurred at 5 mins. A striking finding was that the effect of MAPPs was evident from 5 mins onwards and increased with further incubation in both the phagocytosis and oxidative burst assays. These data suggest that the neutrophilic activity enhanced by PRPr depends more on ATP and ADP during the early phase and on MAPPs during the later phase of the phagocytic process. The present study reveals an important role for PRPr and their phagocytosis activators in the enhancement of neutrophilic activity in human whole blood.

引用

页码：321 / 328

页数：8

共 28 条

[1] ALEXANDER Z, 2007, BLOOD REV, V21, P99
[2] Flow cytometric measurement of phagocytosis reveals a role for C3b in metal particle uptake by phagocytes
Baldwin, L
Flanagan, BF
Hunt, JA
[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2005, 73A (01) : 80 - 85
[3] Release of oxygen metabolites from chemoattractant-stimulated neutrophils is inhibited by resting platelets: Role of extracellular adenosine and actin polymerization
Bengtsson, T
Zalavary, S
Stendahl, O
Grenegard, M
[J]. BLOOD, 1996, 87 (10) : 4411 - 4423
[4] The role of complement C3 opsonization, C5a receptor, and CD14 in E. coli-induced up-regulation of granulocyte and monocyte CD11b/CD18 (CR3), phagocytosis, and oxidative burst in human
Brekke, Ole-Lars
Christiansen, Dorte
Fure, Hilde
Fung, Michael
Mollnes, Tom E.
[J]. JOURNAL OF LEUKOCYTE BIOLOGY, 2007, 81 (06) : 1404 - 1413
[5] Glycosyl-phosphatidyl-inositol-defective granulocytes from paroxysmal nocturnal haemoglobinuria patients show increased bacterial ingestion but reduced respiratory burst induction
Cacciapuoti, Carmela
Terrazzano, Giuseppe
Barone, Lucia
Sica, Michela
Becchimanzi, Cristina
Rotoli, Bruno
Ruggiero, Giuseppina
Alfinito, Fiorella
[J]. AMERICAN JOURNAL OF HEMATOLOGY, 2007, 82 (02) : 98 - 107
[6] Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases
Caron, E
Hall, A
[J]. SCIENCE, 1998, 282 (5394) : 1717 - 1721
[7] POLYMORPHONUCLEAR LEUKOCYTE ADHESION TO ENDOTHELIAL-CELLS IS INHIBITED BY RESTING PLATELETS
DELMASCHIO, A
BAZZONI, G
DEJANA, E
[J]. ARTERIOSCLEROSIS AND THROMBOSIS, 1992, 12 (12): : 1420 - 1427
[8] A FAST AND EASY METHOD TO DETERMINE THE PRODUCTION OF REACTIVE OXYGEN INTERMEDIATES BY HUMAN AND MURINE PHAGOCYTES USING DIHYDRORHODAMINE-123
EMMENDORFFER, A
HECHT, M
LOHMANNMATTHES, ML
ROESLER, J
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 131 (02) : 269 - 275
[9] PROSTAGLANDIN ENDOPEROXIDES - NOVEL TRANSFORMATIONS OF ARACHIDONIC-ACID IN HUMAN PLATELETS
HAMBERG, M
SAMUELSS.B
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (09) : 3400 - 3404
[10] Holmsen H, 1969, Scand J Haematol Suppl, V8, P3

← 1 2 3 →