The role of adipose-derived stromal cells and hydroxypropylmethylcellulose in engineering cartilage tissue in vivo

被引：4
作者：
Xu, YuQiao [1 ,2 ,3 ]
Zhang, Jing [4 ]
Ma, Yu [2 ,3 ]
Han, Yu [1 ]
Min, Jie [2 ,3 ]
Liang, YuanYuan [1 ]
Zhao, DaQing [1 ,2 ,3 ]
Qiu, JianHua [1 ]
机构：
[1] Fourth Mil Med Univ, Xijing Hosp, Dept Otolaryngol Head & Neck Surg, Xian 710032, Shaanxi, Peoples R China
[2] Fourth Mil Med Univ, Xijing Hosp, State Key Lab Canc Biol, Xian 710032, Shaanxi, Peoples R China
[3] Fourth Mil Med Univ, Xijing Hosp, Dept Pathol, Xian 710032, Shaanxi, Peoples R China
[4] Peoples Liberat Army, Dept Nephrol & Endocrinol, Cent Hosp 371, Xinxiang, Henan, Peoples R China
来源：
CYTOTECHNOLOGY | 2014年 / 66卷 / 05期
关键词：
Adipose tissue-derived stromal cells; Hydroxypropylmethylcellulose; Transforming growth factor-beta 1; Basic fibroblast growth factor; Cartilage; Differentiation; MESENCHYMAL STEM-CELLS; ARTICULAR-CARTILAGE; DIFFERENTIATION; REPAIR; CHONDROCYTES; EXPRESSION; HYDROGEL; DEFECTS; INVIVO; VITRO;
D O I：
10.1007/s10616-013-9627-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study demonstrated a newly developed method using adipose tissue-derived stromal cells (ADSCs) and hydroxypropylmethylcellulose (HPMC) in building injectable tissue engineered cartilage in vivo. ADSCs from rabbit subcutaneous fatty tissue were cultured in chondrogenic differentiation medium and supplemented with transforming growth factor-beta 1 (TGF-beta 1) and basic fibroblast growth factor (bFGF). Histological, immunohistochemistry and RT-PCR analysis confirmed that the ADSCs differentiated into chondrocytes following induction. Induced ADSCs mixed with 15 % HPMC were injected into the subcutaneous tissue of nude mice and, after a period of 8 weeks, newly formed cartilage was observed at the site of injection. The ability of ADSCs cultured in the induction medium with TGF-beta 1 and bFGF to differentiate into chondrocytes and construct new cartilage indicates that ADSCs are suitable for use as seed cells in cartilage tissue engineering. HPMC, according to its good water solubility and being able to transform from liquid to solid at body temperature, was found to be an ideal scaffold for tissue engineering.
引用
收藏
页码：779 / 790
页数：12
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