Effect of DC-CIK cell on the proliferation, apoptosis and differentiation of leukemia cells

被引：15

作者：

Qu, Hui-Qing ^{[1
]}

Zhou, Xiao-Sheng ^{[2
]}

Zhou, Xiao-Long ^{[3
]}

Wang, Jian ^{[4
]}

机构：

[1] Binzhou Med Coll, Affiliated Hosp, Dept Blood Transfus, Binzhou 256603, Peoples R China

[2] Binzhou Med Coll, Affiliated Hosp, Dept Neurol, Binzhou 256603, Peoples R China

[3] Binzhou Med Coll, Affiliated Hosp, Dept Pharmaceut, Binzhou 256603, Peoples R China

[4] Binzhou Med Coll, Affiliated Hosp, Dept Lab, Binzhou 256603, Peoples R China

来源：

ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE | 2014年 / 7卷 / 08期

关键词：

Fetal blood; Dendritic cells; Adoptive immunotherapy; HL-60; Immunotherapy; Adoptive; GAMMA;

D O I：

10.1016/S1995-7645(14)60111-5

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Objective: To observe the effect of co-culture cytokine induced killer cells (CIK) and homologous dendritic cells (DC) on the proliferative activity and phenotype change of the DC-CIK cell and the cell killing activity of leukemia HL-60. Methods: 50 mL cord blood sample was obtained from infants delivered by full term healthy woman and the cord blood mononuclear cells were isolated by density gradient centrifugation. Non adherent cells were collected for the induction culture of CIK, adherent cells were differentiated into mature DC; cultured mature DC was mixed with and CIK in the proportion of 1:5 for 12 d. Killing activity of DC-CIK co-cultured cell on leukemia HL-60 was detected by MTT assay. Results: Compared with CIKs, the co- cultured DC CIKs presented a markedly higher proliferation and killing activity. Conclusions: Co-culture of DC-CIK cells led to a significant increase of the proliferation and cytotoxicity of CIK.

引用

页码：659 / 662

页数：4