Real-Time Electrochemical LAMP for DNA Detection of Salmonella spp.

It is important to have clinical testing tools for food safety testing, forensic analysis, homeland security and environmental monitoring in recent years. How to rapidly and accurately find infected pathogens in a limited resource environment is an important topic. In the remote areas of genotoxic infection, Salmonella is one of the most common food borne pathogens. Rapid and accurate identification of Salmonella from food samples by public health agencies and diagnostic laboratories enable better to product quality and effective in preventing outbreaks of infection. In the research, we have developed an electrochemical platform with real-time monitoring LAMP of Salmonella spp. genomic DNA in foods. We have determined 30 mu M methylene blue (MB) as DNA intercalating redox probes to optimal concentration in the LAMP reaction buffer. Then, we have evaluated and valuated the real-time electrochemical LAMP assay for serial dilutions of Salmonella spp. comparing to fluorescence method. Their corresponding melting curves were also implemented. In the electrochemical and fluorescence LAMP, a detection limit as 1 DNA copy of the Salmonella DNA target was achieved in a reaction time less than 30 min.