Cereblon is expressed in the retina and binds to voltage-gated chloride channels

被引：50
作者：
Hohberger, Bettina [1 ]
Enz, Ralf [1 ]
机构：
[1] Univ Erlangen Nurnberg, Emil Fischer Zentrum, Inst Biochem, D-91054 Erlangen, Germany
来源：
FEBS LETTERS | 2009年 / 583卷 / 04期
关键词：
Cereblon; CIC-1; CIC-2; Protein interaction; Retina; CRBN; MENTAL-RETARDATION; CYTOPLASMIC DOMAIN; CRYSTAL-STRUCTURE; BETA-SUBUNIT; LON PROTEASE; RECEPTORS; MUTATION; REVEALS; CELLS; CIC-2;
D O I：
10.1016/j.febslet.2009.01.018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The function of the central nervous system depends on the correct regulation of ion channels by interacting proteins. Here, we identified cereblon as a new interactor of the voltage-gated chloride channel CIC-2. A distal region of the CIC-2 C-terminus interacts with the Lon domain of cereblon. Cereblon is expressed in several brain regions including the retina. There, we detected cereblon in nuclear and synaptic layers and localized the protein in the same subcellular region of bipolar cell bodies previously reported to express CIC-2. Our data suggest that cereblon might be associated with voltage-gated chloride channels in the central nervous system. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：633 / 637
页数：5
相关论文
共 18 条
  • [1] Male germ cells and photoreceptors, both dependent on close cell-cell interactions, degenerate upon ClC-2Cl- channel disruption
    Bösl, MR
    Stein, V
    Hübner, C
    Zdebik, AA
    Jordt, SE
    Mukhopadhyay, AK
    Davidoff, MS
    Holstein, AF
    Jentsch, TJ
    [J]. EMBO JOURNAL, 2001, 20 (06) : 1289 - 1299
  • [2] Alternative mRNA splice variants of the rat CIC-2 chloride channel gene are expressed in lung: genomic sequence and organization of CIC-2
    Chu, SJ
    Zeitlin, PL
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (20) : 4153 - 4159
  • [3] ZIP3, a new splice variant of the PKC-ζ-interacting protein family, binds to GABAc receptors, PKC-ζ, and Kvβ2
    Croci, C
    Brandstätter, JH
    Enz, R
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (08) : 6128 - 6135
  • [4] Role of intramolecular and intermolecular interactions in ClC channel and transporter function
    Dhani, S
    Bear, C
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2006, 451 (06): : 708 - 715
  • [5] X-ray structure of a CIC chloride channel at 3.0 Å reveals the molecular basis of anion selectivity
    Dutzler, R
    Campbell, EB
    Cadene, M
    Chait, BT
    MacKinnon, R
    [J]. NATURE, 2002, 415 (6869) : 287 - 294
  • [6] Enz R, 1999, J NEUROSCI, V19, P9841
  • [7] Barttin is a Cl- channel β-subunit crucial for renal Cl- reabsorption and inner ear K+ secretion
    Estévez, R
    Bottger, T
    Stein, V
    Birkenhäger, R
    Otto, E
    Hildebrandt, F
    Jentsch, TJ
    [J]. NATURE, 2001, 414 (6863) : 558 - 561
  • [8] 3D-Jury: a simple approach to improve protein structure predictions
    Ginalski, K
    Elofsson, A
    Fischer, D
    Rychlewski, L
    [J]. BIOINFORMATICS, 2003, 19 (08) : 1015 - 1018
  • [9] A mutation in a novel ATP-dependent Lon protease gene in a kindred with mild mental retardation
    Higgins, JJ
    Pucilowska, J
    Lombardi, RQ
    Rooney, JP
    [J]. NEUROLOGY, 2004, 63 (10) : 1927 - 1931
  • [10] Dysregulation of large-conductance Ca2+-activated K+ channel expression in nonsyndromal mental retardation due to a cereblon p.R419X mutation
    Higgins, Joseph J.
    Hao, Jin
    Kosofsky, Barry E.
    Rajadhyaksha, Anjali M.
    [J]. NEUROGENETICS, 2008, 9 (03) : 219 - 223
12