Development of a multilocus sequence typing scheme for Ureaplasma

被引：26

作者：

Zhang, J. ^{[1
,2
]}

Kong, Y. ^{[1
,2
]}

Feng, Y. ^{[2
]}

Huang, J. ^{[1
]}

Song, T. ^{[1
,2
]}

Ruan, Z. ^{[2
]}

Song, J. ^{[1
,2
]}

Jiang, Y. ^{[2
]}

Yu, Y. ^{[1
,2
]}

Xie, X. ^{[1
,2
]}

机构：

[1] Zhejiang Univ, Sir Run Run Shaw Hosp, Coll Med, Clin Lab, Hangzhou 310016, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sir Run Run Shaw Hosp, Coll Med, Biomed Res Ctr, Hangzhou 310016, Zhejiang, Peoples R China

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2014年 / 33卷 / 04期

基金：

中国国家自然科学基金;

关键词：

FIELD GEL-ELECTROPHORESIS; GENOTYPIC CHARACTERIZATION; MYCOPLASMA-HOMINIS; PRETERM INFANTS; BIOVARS; UREALYTICUM; IDENTIFICATION; PATHOGEN; STRAINS; PARVUM;

D O I：

10.1007/s10096-013-1981-y

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Ureaplasma is a commensal of the human urogenital tract but is always associated with invasive diseases such as non-gonococcal urethritis and infertility adverse pregnancy outcomes. To better understand the molecular epidemiology and population structure of Ureaplasma, a multilocus sequence typing (MLST) scheme based on four housekeeping genes (ftsH, rpL22, valS, thrS) was developed and validated using 283 isolates, including 14 serovars of reference strains and 269 strains obtained from clinical patients. A total of 99 sequence types (STs) were revealed: the 14 type strains of the Ureaplasma serovars were assigned to 12 STs, and 87 novel and special STs appeared among the clinical isolates. ST1 and ST22 were the predominant STs, which contained 68 and 70 isolates, respectively. Two clonal lineages (CC1 and CC2) were shown by eBURST analysis, and linkage disequilibrium was revealed through a standardized index of association (I (A) (S)). The neighbor-joining tree results of 14 Ureaplasma serovars showed two genetically significantly distant clusters, which was highly congruent with the species taxonomy of ureaplasmas [Ureaplasma parvum (UPA) and Ureaplasma urealyticum (UUR)]. Analysis of the biotypes of 269 clinical isolates revealed that all the isolates of CC1 were UPA and those of CC2 were UUR. Additionally, CC2 was found more often in symptomatic patients with vaginitis, tubal obstruction, and cervicitis. In conclusion, this MLST scheme is adequate for investigations of molecular epidemiology and population structure with highly discriminating capacity.

引用

页码：537 / 544

页数：8

共 33 条

[1] Association of Ureaplasma urealyticum biovars with clinical outcome for neonates, obstetric patients, and gynecological patients with pelvic inflammatory disease [J].