Genetic fingerprinting of Pseudomonas syringae pathovars using ERIC-, REP-, and IS50-PCR

被引:55
作者
Weingart, H
Volksch, B
机构
来源
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT | 1997年 / 145卷 / 8-9期
关键词
D O I
10.1111/j.1439-0434.1997.tb00411.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
PCR fingerprinting using primers corresponding to repetitive (ERIC and REP) and insertion sequences (IS50) was investigated as a method to distinguish the pathovars of Pseudomonas syringae. After amplification of total DNA with the ERIC-, REP-, and IS50-PCR followed by agarose gel electrophoresis, most of the tested pathovars showed specific patterns of PCR products. The differences between the fingerprints among strains within a pathovar were small, with the exception of pathovars syringae, aptata, and atrofaciens. The fingerprints of the related pathovars savastanoi, phaseolicola, glycinea, morsprunorum, tabaci, lachrymans, and mori generated with the ERIC- and REP-primers were found to be very similar, showing the potential of this technique for taxonomical studies. In contrast, the IS50-PCR fingerprints of these pathovars were clearly distinguishable. The fingerprint patterns of a strain were highly reproducible with all three tested primer sets, also when whole cells were added to the reaction mixture. Thus, the PCR technique with the ERIC-, REP-, and IS50-primers is a rapid, simple, reproducible, and low cost method to identify and classify strains of the Pseudomonas syringae pathovars.
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页码:339 / 345
页数:7
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