Injectable Photo-Crosslinked Bioactive BMSCs-BMP2-GelMA Scaffolds for Bone Defect Repair

被引：22
|
作者：
Chai, Senlin [1 ]
Huang, Jianhao [2 ]
Mahmut, Abdurahman [1 ]
Wang, Bin [3 ]
Yao, Yao [1 ]
Zhang, Xiaofeng [1 ]
Zhuang, Zaikai [1 ]
Xie, Chunmei [4 ]
Xu, Zhihong [1 ]
Jiang, Qing [1 ,5 ]
机构：
[1] Affiliated Hosp Nanjing Univ Med Sch, Nanjing Drum Tower Hosp, Dept Orthoped Surg, Div Sports Med & Adult Reconstruct Surg,State Key, Nanjing, Peoples R China
[2] Southern Med Univ, Jinling Hosp, Sch Clin Med 1, Dept Orthoped, Nanjing, Peoples R China
[3] First Affiliated Hosp Zhejiang Univ Sch Med, Hangzhou, Peoples R China
[4] Hangzhou Lancet Robot Co Ltd, Hangzhou, Peoples R China
[5] Jiangsu Engn Res Ctr 3D Bioprinting, Nanjing, Peoples R China
来源：
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY | 2022年 / 10卷
基金：
中国博士后科学基金;
关键词：
GelMA; BMSCs; BMP2; scaffold; photo-crosslinked; osteogenic differentiation; bone defect; MESENCHYMAL STEM-CELLS; OSTEOGENIC DIFFERENTIATION; MORPHOGENETIC PROTEIN-2; HYDROGEL; MICROSPHERES; BMP-2; SPINE;
D O I：
10.3389/fbioe.2022.875363
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Injectable hydrogels offer a new therapy option for irregular bone deformities. Based on gelatin methacryloyl (GelMA), bone marrow mesenchymal stem cells (BMSCs), and bone morphogenetic protein 2 (BMP2), we created a photo-crosslinked composite bioactive scaffold. The composite scaffolds had appropriate mechanical properties for stem cells adhesion and proliferation, as well as good biocompatibility and the ability to stimulate BMSCs osteogenic differentiation in vitro. The synergistic effect of BMSCs and BMP2 enabled the composite bioactive scaffold to exhibit higher osteogenic potential in vivo than scaffolds loaded alone with BMSCs or BMP2, according to imaging and histology studies. In conclusion, by promoting the osteogenic differentiation of BMSCs, the composite bioactive scaffold based on BMSCs-BMP2-GelMA has demonstrated remarkable application potential in bone regeneration and bone defects repair.
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页数：11
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