Functional Characterization of the Pacific Oyster, Crassostrea gigas (Bivalvia: Ostreidae), Hemocytes Under Normoxia and Short-Term Hypoxia

被引：17

作者：

Andreyeva, Aleksandra Yu ^{[1
]}

Kladchenko, Ekaterina S. ^{[1
]}

Vyalova, Oksana Y. ^{[1
]}

Kukhareva, Tatiana A. ^{[1
]}

机构：

[1] RAS, Moscow Representat Off AO Kovalevsky Inst Biol So, Dept Anim Physiol & Biochem, Leninsky Ave 38, Moscow 119991, Russia

来源：

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES | 2021年 / 21卷 / 03期

关键词：

Crassostrea gigas; Hypoxia; Hemocytes; MORPHOLOGICAL CHARACTERIZATION; FLOW-CYTOMETRY; ACIDIFICATION; PHAGOCYTOSIS; PARAMETERS; RESPONSES; CELLS; LIGHT; CLAM;

D O I：

10.4194/1303-2712-v21_3_03

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Hemolymph cellular composition, morphology and functional properties of the Pacific oyster (Crassostrea gigas) hemocytes were studied. Three hemocyte types (agranulocytes, hyalinocytes and granulocytes) were described in hemolymph. The morphology of each type was characterized by light microscopy and flow cytometry. Agranular cells (agranulocytes and hyalinocytes) were the dominant type of cells in hemolymph; their number was 86.7 +/- 2.7% of total cell count. Under hypoxia the number of agranulocytes increased (37.4% for control group versus 95.3% for hypoxic probes), whereas granulocyte and hyalinocyte number decreased up to 3.9% and 0.7% in hypoxic specimens respectively. The spontaneous ROS production decreased in each hemocyte type after exposure to hypoxia. Low dissolved oxygen did not influence hemocyte proliferation and mortality level.

引用

页码：125 / 133

页数：9

共 63 条

[1] Fish response to hypoxia stress: growth, physiological, and immunological biomarkers [J].

Abdel-Tawwab, Mohsen ;

Monier, Mohamed N. ;

Hoseinifar, Seyed Hossein ;

Faggio, Caterina .

FISH PHYSIOLOGY AND BIOCHEMISTRY, 2019, 45 (03) :997-1013

[2] Sydney rock oyster (Saccostrea glomerata) hemocytes:: Morphology and function [J].

Aladaileh, Saleem ;

Nair, Sham V. ;

Birch, Debra ;

Raftos, David A. .

JOURNAL OF INVERTEBRATE PATHOLOGY, 2007, 96 (01) :48-63

[3] Flow cytometric comparison of haemocytes from three species of bivalve molluscs [J].

Allam, B ;

Ashton-Alcox, KA ;

Ford, SE .

FISH & SHELLFISH IMMUNOLOGY, 2002, 13 (02) :141-158

[4] Morphological and functional characterization of hemocytes in cultivated mussel (Mytilus galloprovincialis) and effect of hypoxia on hemocyte parameters [J].

Andreyeva, A. Y. ;

Efremova, E. S. ;

Kukhareva, T. A. .

FISH & SHELLFISH IMMUNOLOGY, 2019, 89 :361-367

[5] Black Scorpionfish (Scorpaena porcus) Hemopoiesis: Analysis by Flow Cytometry and Light Microscopy [J].

Andreyeva, Aleksandra Y. ;

Soldatov, Aleksander A. ;

Kukhareva, Tatiana A. .

ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY, 2017, 300 (11) :1993-1999

[6] SEPARATION OF CRASSOSTREA-GIGAS HEMOCYTES BY DENSITY GRADIENT CENTRIFUGATION AND COUNTERFLOW CENTRIFUGAL ELUTRIATION [J].

BACHERE, E ;

CHAGOT, D ;

GRIZEL, H .

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1988, 12 (03) :549-559

[7] Variability in Atlantic water temperature and transport at the entrance to the Arctic Ocean, 1997-2010 [J].

Beszczynska-Moeller, Agnieszka ;

Fahrbach, Eberhard ;

Schauer, Ursula ;

Hansen, Edmond .

ICES JOURNAL OF MARINE SCIENCE, 2012, 69 (05) :852-863

[8]

Boyd JN, 1999, J EXP BIOL, V202, P3135

[9] THE DYNAMICS AND IMPACT OF OCEAN ACIDIFICATION AND HYPOXIA Insights from Sustained Investigations in the Northern California Current Large Marine Ecosystem [J].

Chan, Francis ;

Barth, John A. ;

Kroeker, Kristy ;

Lubchenco, Jane ;

Menge, Bruce A. .

OCEANOGRAPHY, 2019, 32 (03) :62-71

[10]

Chang SJ, 2005, ZOOL STUD, V44, P144

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