Iron-limited condition modulates biofilm formation and interaction with human epithelial cells of enteroaggregative Escherichia coli (EAEC)

被引：15

作者：

Alves, J. R. ^{[1
]}

Pereira, A. C. M. ^{[1
]}

Souza, M. C. ^{[1
]}

Costa, S. B. ^{[1
]}

Pinto, A. S. ^{[1
]}

Mattos-Guaraldi, A. L. ^{[1
]}

Hirata-Junior, R. ^{[1
]}

Rosa, A. C. P. ^{[1
]}

Asad, L. M. B. O. ^{[2
]}

机构：

[1] Univ Estado Rio De Janeiro, Dept Microbiol Imunol Parasitol, Fac Ciencias Med, Rio De Janeiro, Brazil

[2] Univ Estado Rio De Janeiro, Dept Biofis & Biometria, Inst Biol Roberto Alcantara Gomes, Rio De Janeiro, Brazil

来源：

JOURNAL OF APPLIED MICROBIOLOGY | 2010年 / 108卷 / 01期

关键词：

2; 2-dipyridyl; biofilm; EAEC; HEp-2; cell; iron; LIMITATION; ADHERENCE; GENES;

D O I：

10.1111/j.1365-2672.2009.04417.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: The aim of this study was to investigate the influence of low iron availability on biofilm formation and adherence to HEp-2 cells of enteroaggregative Escherichia coli (EAEC) strains isolated from diarrhoea cases. Methods and Results: The ability of EAEC to form biofilm on a plastic surface was evaluated quantitatively and qualitatively after 3 and 18 h of incubation of strains with or without the iron chelator 2,2-dipyridyl. When submitted to low iron conditions, prototype EAEC 042 strain showed a decrease in biofilm formation. Conversely, an increase in biofilm formation was observed for the clinical EAEC strains cultured in restricted iron condition. Moreover, the reduction of iron concentration inhibited the aggregative adherence to HEp-2 cells of all EAEC strains tested. However, all effects promoted by iron chelation were suppressed by thiourea. Conclusions: Low iron availability may modulate biofilm formation and adhesive properties of EAEC strains to HEp-2 cells. Significance and Impact of the Study: The data obtained in this study provide useful insights on the influence of low iron conditions possibly associated with redox stress on the pathogenesis of EAEC strains.

引用

页码：246 / 255

页数：10

共 30 条

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