Recombinant hemoglobins as artificial oxygen carriers

被引：9

作者：

Fronticelli, Clara

Koehler, Raymond C.

Brinigar, William S.

机构：

[1] Johns Hopkins Univ, Dept Anesthesiol & Crit Care Med, Sch Med, Baltimore, MD 21287 USA

[2] Temple Univ, Dept Chem, Philadelphia, PA 19122 USA

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2007年 / 35卷 / 01期

关键词：

blood substitute; heme pocket mutants; molecular engineering; polymeric hemoglobin; surface mutant;

D O I：

10.1080/10731190600974467

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This paper describes the approaches we have taken to construct a) mutant hemoglobins with different oxygen affinities, and b) mutant hemoglobins and myoglobins that polymerize to high molecular weight aggregates in an effort to prevent extravasation and the associated vasoactivity. In vivo testing indicates that exchange transfusion of polymeric hemoglobins in mice does not result in vasoactivity and that polymeric hemoglobins are effective oxygen carriers to ischemic tissues irrespective of their oxygen affinity and cooperativity.

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页码：45 / 52

页数：8

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