HuB and HuD repress telomerase activity by dissociating HuR from TERC

被引：11
作者：
Cheng, Xiaolei [1 ,2 ,3 ]
Gu, Xiaoping [4 ]
Xia, Tianjiao [4 ]
Ma, Zhengliang [4 ]
Yang, Zhongzhou [5 ,6 ]
Feng, Helen Lechen [7 ]
Zhao, Yong [8 ]
Ma, Wenbin [8 ]
Ju, Zhenyu [9 ]
Gorospe, Myriam [10 ]
Yi, Xia [1 ]
Tang, Hao [2 ,3 ]
Wang, Wengong [1 ,11 ]
机构：
[1] Peking Univ Hlth Sci Ctr, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing Key Lab Prot Posttranslat Modificat & Cel, 38 Xueyuan Rd, Beijing 100191, Peoples R China
[2] Zhengzhou Univ, Cent China Fuwai Hosp, Henan Prov Peoples Hosp, Heart Ctr,Key Lab Cardiovasc Regenerat Med,Natl H, Zhengzhou 450003, Henan, Peoples R China
[3] Natl Ctr Cardiovasc Dis, Cent China Branch, Zhengzhou 450003, Henan, Peoples R China
[4] Nanjing Univ, Dept Anesthesiol, Affiliated Drum Tower Hosp, Med Dept, Nanjing 210000, Peoples R China
[5] Nanjing Univ, Model Anim Res Ctr, Nanjing Biomed Res Inst, State Key Lab Pharmaceut Biotechnol, Nanjing 210061, Peoples R China
[6] Nanjing Univ, Model Anim Res Ctr, Nanjing Biomed Res Inst, MOE Key Lab Model Anim Dis Study, Nanjing 210061, Peoples R China
[7] Boston Univ, Dept Biol, 5 Cummington Mall, Boston, MA 02215 USA
[8] Sun Yat Sen Univ, Sch Life Sci, Key Lab Gene Engn, State Key Lab Biocontrol,Minist Educ, Guangzhou 510006, Peoples R China
[9] Jinan Univ, Inst Aging & Regenerat Med, Key Lab Regenerat Med, Minist Educ, Guangzhou 510632, Peoples R China
[10] NIA, Lab Genet & Genom, NIH, 251 Bayview Blvd, Baltimore, MD 21224 USA
[11] Changzhi Med Coll, Ctr Hlth Aging, Changzhi 046000, Peoples R China
来源：
NUCLEIC ACIDS RESEARCH | 2021年 / 49卷 / 05期
关键词：
RNA-BINDING PROTEINS; ELAV; INTERACTS; TPP1; POT1;
D O I：
10.1093/nar/gkab062
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ubiquitous RNA-binding protein HuR (ELAVL1) promotes telomerase activity by associating with the telomerase noncoding RNA TERC. However, the role of the neural-specific members HuB, HuC, and HuD (ELAVL2-4) in telomerase activity is unknown. Here, we report that HuB and HuD, but not HuC, repress telomerase activity in human neuroblastoma cells. By associating with AU-rich sequences in TERC, HuB and HuD repressed the assembly of the TERT-TERC core complex. Furthermore, HuB and HuD competed with HuR for binding to TERC and antagonized the function of HuR that was previously shown to enhance telomerase activity to promote cell growth. Our findings reveal a novel mechanism controlling telomerase activity in human neuroblastoma cells that involves a competition between HuR and the related, neural-specific proteins HuB and HuD.
引用
收藏
页码：2848 / 2858
页数：11
相关论文
共 35 条
  • [21] Human H/ACA small nucleolar RNPs and telomerase share evolutionarily conserved proteins NHP2 and NOP10
    Pogacic, V
    Dragon, F
    Filipowicz, W
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (23) : 9028 - 9040
  • [22] Posttranscriptional regulation of gene expression in learning by the neuronal ELAV-like mRNA-stabilizing proteins
    Quattrone, A
    Pascale, A
    Nogues, X
    Zhao, WQ
    Gusev, P
    Pacini, A
    Alkon, DL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (20) : 11668 - 11673
  • [23] Regulation of human telomerase in homeostasis and disease
    Roake, Caitlin M.
    Artandi, Steven E.
    [J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2020, 21 (07) : 384 - 397
  • [24] Telomere dysfunction induces metabolic and mitochondrial compromise
    Sahin, Erguen
    Colla, Simona
    Liesa, Marc
    Moslehi, Javid
    Mueller, Florian L.
    Guo, Mira
    Cooper, Marcus
    Kotton, Darrell
    Fabian, Attila J.
    Walkey, Carl
    Maser, Richard S.
    Tonon, Giovanni
    Foerster, Friedrich
    Xiong, Robert
    Wang, Y. Alan
    Shukla, Sachet A.
    Jaskelioff, Mariela
    Martin, Eric S.
    Heffernan, Timothy P.
    Protopopov, Alexei
    Ivanova, Elena
    Mahoney, John E.
    Kost-Alimova, Maria
    Perry, Samuel R.
    Bronson, Roderick
    Liao, Ronglih
    Mulligan, Richard
    Shirihai, Orian S.
    Chin, Lynda
    DePinho, Ronald A.
    [J]. NATURE, 2011, 470 (7334) : 359 - 365
  • [25] Antidepressant-like actions by silencing of neuronal ELAV-like RNA-binding proteins HuB and HuC in a model of depression in male mice
    Sanna, Maria Domenica
    Quattrone, Alessandro
    Galeotti, Nicoletta
    [J]. NEUROPHARMACOLOGY, 2018, 135 : 444 - 454
  • [26] Telomerase, senescence and ageing
    Shawi, May
    Autexier, Chantal
    [J]. MECHANISMS OF AGEING AND DEVELOPMENT, 2008, 129 (1-2) : 3 - 10
  • [27] Telomeres and telomerase: three decades of progress
    Shay, Jerry W.
    Wright, Woodring E.
    [J]. NATURE REVIEWS GENETICS, 2019, 20 (05) : 299 - 309
  • [28] HUD, A PARANEOPLASTIC ENCEPHALOMYELITIS ANTIGEN, CONTAINS RNA-BINDING DOMAINS AND IS HOMOLOGOUS TO ELAV AND SEX-LETHAL
    SZABO, A
    DALMAU, J
    MANLEY, G
    ROSENFELD, M
    WONG, E
    HENSON, J
    POSNER, JB
    FURNEAUX, HM
    [J]. CELL, 1991, 67 (02) : 325 - 333
  • [29] Tang H., 2015, AGING CLIN EXP RES
  • [30] HuR regulates telomerase activity through TERC methylation
    Tang, Hao
    Wang, Hu
    Cheng, Xiaolei
    Fan, Xiuqin
    Yang, Fan
    Zhang, Mengmeng
    Chen, Yanlian
    Tian, Yuyang
    Liu, Cihang
    Shao, Dongxing
    Jiang, Bin
    Dou, Yali
    Cong, Yusheng
    Xing, Junyue
    Zhang, Xiaotian
    Yi, Xia
    Zhou Songyang
    Ma, Wenbin
    Zhao, Yong
    Wang, Xian
    Ma, Jinbiao
    Gorospe, Myriam
    Ju, Zhenyu
    Wang, Wengong
    [J]. NATURE COMMUNICATIONS, 2018, 9
1234