Flow cytometry as a tool to identify Mycobacterium tuberculosis interaction with the immune system and drug susceptibility

被引：0

作者：

Bonecini-Almeida, MD ^{[1
]}

机构：

[1] Ctr Pesquisa Hosp Evandro Chagas, Fdn Oswaldo Cruz, Dept Microbiol & Immunol, Cellular Immunol Lab, BR-21045900 Rio De Janeiro, RJ, Brazil

来源：

MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2000年 / 95卷 / 04期

关键词：

tuberculosis; macrophages; drug susceptibility; cytometry;

D O I：

暂无

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Flow cytometric analysis is a useful and widely employed tool to identify immunological alterations caused by different microorganisms, including Mycobacterium tuberculosis. However; this tool can be used for several others analysis. We will discuss some applications for flow cytometry to the study of M. tuberculosis, mainly on cell surface antigens, mycobacterial secreted proteins, their interaction with the immune system using inflammatory cells recovered from peripheral blood, alveolar and pleura spaces and the influence of M. tuberculosis on apoptosis, and finally the rapid determination of drug susceptibility. All of these examples highlight the usefulness of flow cytometry in the study of M. tuberculosis infection.

引用

页码：491 / 494

页数：4

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