Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses

被引：927

作者：

Lavin, Yonit ^{[1
,2
,3
]}

Kobayashi, Soma ^{[1
,2
,3
]}

Leader, Andrew ^{[1
,2
,3
]}

Amir, El-ad David ^{[2
,3
,9
]}

Elefant, Naama ^{[10
]}

Bigenwald, Camille ^{[1
,2
,3
]}

Remark, Romain ^{[1
,2
,3
,13
]}

Sweeney, Robert ^{[6
,7
]}

Becker, Christian D. ^{[4
]}

Levine, Jacob H. ^{[11
]}

Meinhof, Klaus ^{[4
]}

Chow, Andrew ^{[1
,2
,3
]}

Kim-Shulze, Seunghee ^{[2
,3
,9
]}

Wolf, Andrea ^{[6
]}

Medaglia, Chiara ^{[10
]}

Li, Hanjie ^{[10
]}

Rytlewski, Julie A. ^{[12
]}

Emerson, Ryan O. ^{[12
]}

Solovyov, Alexander ^{[1
,3
,5
,8
]}

Greenbaum, Benjamin D. ^{[1
,3
,5
,8
]}

Sanders, Catherine ^{[12
]}

Vignali, Marissa ^{[12
]}

Beasley, Mary Beth ^{[8
]}

Flores, Raja ^{[6
]}

Gnjatic, Sacha ^{[2
,3
,5
,9
]}

Pe'er, Dana ^{[11
]}

Rahman, Adeeb ^{[2
,3
,7
,9
]}

Amit, Ido ^{[10
]}

Merad, Miriam ^{[1
,2
,3
,9
]}

机构：

[1] Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY 10029 USA

[2] Icahn Sch Med Mt Sinai, Precis Immunol Inst, New York, NY 10029 USA

[3] Icahn Sch Med Mt Sinai, Tisch Canc Inst, New York, NY 10029 USA

[4] Icahn Sch Med Mt Sinai, Div Pulmonol, New York, NY 10029 USA

[5] Icahn Sch Med Mt Sinai, Div Hematol Oncol, New York, NY 10029 USA

[6] Icahn Sch Med Mt Sinai, Dept Thorac Surg, New York, NY 10029 USA

[7] Icahn Sch Med Mt Sinai, Dept Genet & Genom Sci, New York, NY 10029 USA

[8] Icahn Sch Med Mt Sinai, Dept Pathol, New York, NY 10029 USA

[9] Icahn Sch Med Mt Sinai, Human Immune Monitoring Ctr, New York, NY 10029 USA

[10] Weizmann Inst Sci, Dept Immunol, IL-76100 Rehovot, Israel

[11] Sloan Kettering Inst, Computat & Syst Biol Program, New York, NY 10065 USA

[12] Adapt Biotechnol Inc, Seattle, WA 98102 USA

[13] Innate Pharma, 117 Ave Luminy, F-13009 Marseille, France

来源：

CELL | 2017年 / 169卷 / 04期

关键词：

TERTIARY LYMPHOID STRUCTURES; FLOW-CYTOMETRIC ANALYSIS; DENDRITIC CELLS; MYELOID CELLS; GENE-EXPRESSION; T-CELLS; RNA-SEQ; CANCER; MACROPHAGES; MONOCYTES;

D O I：

10.1016/j.cell.2017.04.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell analysis of the tumor, noninvolved lung, and blood cells, we provide a detailed immune cell atlas of early lung tumors. We show that stage I lung adenocarcinoma lesions already harbor significantly altered T cell and NK cell compartments. Moreover, we identified changes in tumor-infiltrating myeloid cell (TIM) subsets that likely compromise anti-tumor T cell immunity. Paired single-cell analyses thus offer valuable knowledge of tumor-driven immune changes, providing a powerful tool for the rational design of immune therapies.

引用

页码：750 / 765

页数：16

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