Evaluation of enterovirus recovery in surface water by different adsorption and elution procedures

被引：26

作者：

Hsu, Bing-Mu

Chen, Chien-Hsien ^{[1
]}

Kung, Chien-Min

Wan, Min-Tao

Shen, Shu-Min

机构：

[1] Tatung Univ, Dept Bioengn, Taipei, Taiwan

[2] Yuanpei Univ Sci & Technol, Dept Med Technol, Hsinchu, Taiwan

[3] Natl Chung Cheng Univ, Dept Earth & Environm Engn, Chiayi, Taiwan

[4] Natl Yunlin Univ Sci & Technol, Inst Safety Hlth & Environm Engn, Yunlin, Taiwan

来源：

CHEMOSPHERE | 2007年 / 66卷 / 05期

关键词：

enteroviruses; MPN-RT-PCR; real-time RT-PCR;

D O I：

10.1016/j.chemosphere.2006.06.054

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The performance of six concentration method combinations and two quantitative analysis techniques were evaluated in terms of enterovirus recovery efficiencies by adjusting the pH and salt concentration of water samples. Of the six concentration method combinations, adsorption on nitrocellulose membranes followed by an acid rinse elution consistently gave the highest recovery efficiencies. In theory, an electropositive membrane should be the most appropriate technique for adsorption of electronegative viruses in pure water. However, it displayed the greatest loss in natural waters. For adsorption and elution procedures, both the electronegative membrane, accompanied by an acid rinse step, and the electropositive membrane, accompanied by a glycine rinse step, provided higher recovery efficiencies. MPN-RT-PCR, a statistically quantitative analysis method, performed more efficiently, in economic terms, but had a similar enterovirus recovery trend to real-time RT-PCR, which is the authoritative quantitative analysis method for nucleic acid. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：964 / 969

页数：6

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