Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

被引：101

作者：

Binh Thanh Vu ^{[1
,6
]}

Shahin, Sophia Allaf ^{[2
]}

Croissant, Jonas ^{[3
,7
]}

Fatieiev, Yevhen ^{[3
]}

Matsumoto, Kotaro ^{[4
]}

Doan, Tan Le-Hoang ^{[4
,8
]}

Yik, Tammy ^{[1
]}

Simargi, Shirleen ^{[2
]}

Conteras, Altagracia ^{[2
]}

Ratliff, Laura ^{[2
]}

Jimenez, Chiara Mauriello ^{[5
]}

Raehm, Laurence ^{[5
]}

Khashab, Niveen ^{[3
]}

Durand, Jean-Olivier ^{[5
]}

Glackin, Carlotta ^{[2
]}

Tamanoi, Fuyuhiko ^{[1
,4
]}

机构：

[1] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90024 USA

[2] City Hope Natl Med Ctr, Beckman Res Inst, Dept Dev & Stem Cell Biol, Duarte, CA USA

[3] King Abdullah Univ Sci & Technol, Smart Hybrid Mat Lab SHMs, Thuwal, Saudi Arabia

[4] Kyoto Univ, Inst Adv Study, Inst Integrated Cell Mat Sci, Kyoto, Japan

[5] UM1, UM2, Inst Charles Gerhardt Montpellier, CNRS,ENSCM,UMR 5253, Montpellier, France

[6] Vietnam Natl Univ Ho Chi Minh City, Lab Stem Cell Res & Applicat, Ho Chi Minh City, Vietnam

[7] Univ New Mexico, Ctr Microengn Mat, Adv Mat Lab, Albuquerque, NM USA

[8] Vietnam Natl Univ Ho Chi Minh City, Ctr Innovat Mat & Architectures, Ho Chi Minh City, Vietnam

来源：

SCIENTIFIC REPORTS | 2018年 / 8卷

基金：

美国国家科学基金会;

关键词：

MESOPOROUS SILICA NANOPARTICLES; CONTROLLED-RELEASE; ORGANOSILICA NANOPARTICLES; DELIVERY; EMBRYO; THERAPY; BIOCOMPATIBILITY; XENOGRAFT; SIRNA; CAM;

D O I：

10.1038/s41598-018-25573-8

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.

引用

页数：10