The efficient synthesis and purification of amyloid-β(1-42) using an oligoethylene glycol-containing photocleavable lysine tag

被引：11
|
作者：
Karas, John A. [1 ,2 ,3 ]
Noor, Asif [1 ,2 ]
Schieber, Christine [1 ,2 ]
Connell, Timothy U. [1 ,2 ]
Separovic, Frances [1 ,2 ]
Donnelly, Paul S. [1 ,2 ]
机构：
[1] Univ Melbourne, Sch Chem, Melbourne, Vic 3010, Australia
[2] Univ Melbourne, Bio21 Mol Sci & Biotechnol Inst, Melbourne, Vic 3010, Australia
[3] Univ Melbourne, Dept Chem & Biomol Engn, Melbourne, Vic 3010, Australia
来源：
CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 51期
基金：
澳大利亚研究理事会;
关键词：
D O I：
10.1039/c7cc03147e
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A short, monodisperse oligoethylene glycol-containing photocleavable lysine tag was developed to facilitate the efficient purification of hydrophobic and fibril-forming peptides. This new tag was used to prepare a modified A beta(42) peptide with increased solubility and decreased propensity to aggregate in aqueous media. The solubilising tag was readily removed by irradiation with UV light and permitted the preparation and isolation of A beta(42) in high purity and yield.
引用
收藏
页码：6903 / 6905
页数：3
相关论文
共 25 条
  • [1] Solid-phase synthesis and purification of a-amyloid (1-42)
    Sidorova, M. V.
    Molokoedov, A. S.
    Ovchinnikov, M. V.
    Bespalova, Z. D.
    Mendeleev Communications, 1996, (06)
  • [2] Prevention of Amyloid-β Fibril Formation Using Antibodies Against the C-terminal Region of Amyloid-β1-40 and Amyloid-β1-42
    Montanes, Maria
    Casabona, Diego
    Sarasa, Leticia
    Pesini, Pedro
    Sarasa, Manuel
    JOURNAL OF ALZHEIMERS DISEASE, 2013, 34 (01) : 133 - 137
  • [3] Electrochemical Immunosensor for the Sensitive Detection of Alzheimer's Biomarker Amyloid-β (1-42) Using the Heme-amyloid-β (1-42) Complex as the Signal Source
    Ding, Minling
    Shu, Qi
    Zhang, Nan
    Yan, Chaoren
    Niu, Huizhe
    Li, Xiaoqian
    Guan, Ping
    Hu, Xiaoling
    ELECTROANALYSIS, 2022, 34 (02) : 263 - 274
  • [4] Solid-phase synthesis and purification of beta-amyloid (1-42)
    Sidorova, MV
    Molokoedov, AS
    Ovchinnikov, MV
    Bespalova, ZD
    Kudryavtseva, EV
    Rubina, AY
    Bushuev, VN
    Schmidt, M
    Klose, A
    MENDELEEV COMMUNICATIONS, 1996, (06) : 232 - 235
  • [5] Isolation and analysis of amyloid-β 1-42 monomer and oligomers in liquid droplets using an immunoaffinity membrane
    Shimazaki, Youji
    Hirose, Yu
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2014, 972 : 53 - 57
  • [6] Analysis of the stabilities of hexameric amyloid-β(1-42) models using discrete molecular dynamics simulations
    Yun, Sijung
    Yun, Sajung
    Guy, H. Robert
    JOURNAL OF MOLECULAR GRAPHICS & MODELLING, 2011, 29 (05): : 657 - 662
  • [7] Anti-amyloidogenic Approach to Access Amyloid-(1-42) in Fmoc Solid-Phase Synthesis
    Lee, Sejin
    Kim, YoungSoo
    BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2015, 36 (08) : 2147 - 2149
  • [8] Efficient Direct Solid-Phase Synthesis of β-Amyloid (1-42) Peptide Using Controlled Microwave Irradiation
    Bacsa, B.
    Bosze, S.
    Kappe, C. O.
    JOURNAL OF PEPTIDE SCIENCE, 2010, 16 : 80 - 80
  • [9] A method for the detection of amyloid-β1-40, amyloid-β1-42 and amyloid-β oligomers in blood using magnetic beads in combination with flow cytometry and its application in the diagnostics of Alzheimer's disease
    Santos, Alexander Navarrete
    Simm, Andreas
    Holthoff, Vjera
    Boehm, Gerald
    JOURNAL OF ALZHEIMERS DISEASE, 2008, 14 (02) : 127 - 131
  • [10] An electrochemical peptide-based biosensor for the Alzheimer biomarker amyloid-β(1-42) using a microporous gold nanostructure
    Negahdary, Masoud
    Heli, Hossein
    MICROCHIMICA ACTA, 2019, 186 (12)
123