DFTMP, an NMR reagent for assessing the near-neutral pH of biological samples

被引：31

作者：

Reily, Michael D. ^{[1
]}

Robosky, Lora C. ^{[1
]}

Manning, Matthew L. ^{[1
]}

Butler, Andrew ^{[1
]}

Baker, John David ^{[1
]}

Winters, R. Thomas ^{[1
]}

机构：

[1] Pfizer Global Res & Dev, Ann Arbor, MI 48105 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 38期

关键词：

D O I：

10.1021/ja063773h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new NMR chemical shift standard and pH indicator, difluorotrimethylsilanylphosphonic acid (DFTMP), is described, and the utility of this reagent is demonstrated for in situ determination of pH in complex biofluids. The pH dependence of this reagent allows accurate in situ determination of aqueous solution pH to within an RMSE of 0.02 pH units over a pH range of 5 to 8. Advantages of this reagent over previously described pH-sensitive components include (1) lack of metal binding affinity, (2) minimal disturbance of endogenous spectral regions, and (3) the potential to function as a multinuclear pH indicator and chemical shift reference point for 19F, 1H, and 31P nuclei. This reagent will be generally useful for NMR experiments on biological systems where the pH needs to be accurately measured at the moment of data acquisition. Copyright © 2006 American Chemical Society.

引用

页码：12360 / 12361

页数：2

共 9 条

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