Bacterial polysaccharide levan as stabilizing, non-toxic and functional coating material for microelement-nanoparticles

被引：52
作者：
Bondarenko, Olesja M. [1 ]
Ivask, Angela [1 ]
Kahru, Anne [1 ]
Vija, Heiki [1 ]
Titma, Tiina [1 ]
Visnapuu, Meeri [1 ,2 ]
Joost, Urmas [2 ]
Pudova, Ksenia [3 ,4 ]
Adamberg, Signe [4 ]
Visnapuu, Triinu [5 ]
Alamaee, Tiina [5 ]
机构：
[1] NICPB, Lab Environm Toxicol, Akad Tee 23, EE-12618 Tallinn, Estonia
[2] Univ Tartu, Inst Phys, EE-50411 Tartu, Estonia
[3] Tallinn Univ Technol, EE-19086 Tallinn, Estonia
[4] Competence Ctr Food & Fermentat Technol, EE-12618 Tallinn, Estonia
[5] Univ Tartu, Inst Mol & Cell Biol, EE-51010 Tartu, Estonia
来源：
CARBOHYDRATE POLYMERS | 2016年 / 136卷
关键词：
Safe-by-design; Food supplement; Co; Fe and Se nanomaterial; Caco-2 in vitro; Bacteroides thetaiotaomicron; CELLS IN-VITRO; HUMAN GUT; ANTITUMOR-ACTIVITY; TEST ORGANISMS; HUMAN-COLON; TOXICITY; LEVANSUCRASES; SPECIFICITY; STRATEGIES; CATALYSTS;
D O I：
10.1016/j.carbpol.2015.09.093
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Levan, fructose-composed biopolymer of bacterial origin, has potential in biotechnology due to its prebiotic and immunostimulatory properties. In this study levan synthesized by levansucrase from Pseudomonas syringae was thoroughly characterized and used as multifunctional biocompatible coating material for microelement-nanoparticles (NPs) of selenium, iron and cobalt. Transmission electron microscopy (TEM), hydrodynamic size measurements (DLS) and X-ray photoelectron spectroscopy (XPS) showed the interaction of levan with NPs. Levan stabilized the dispersions of NPs, decreased their toxicity and had protective effect on human intestinal cells Caco-2. In addition, levan attached to cobalt NPs remained accessible as a substrate for the colon bacteria Bacteroides thetaiotaomicron. We suggest that the combination of levan and nutritionally important microelements in the form of NPs serves as a first step towards a novel "2 in 1" approach for food supplements to provide safe and efficient delivery of microelements for humans and support beneficial gut microbiota with nutritional oligosaccharides. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：710 / 720
页数：11
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