Arabidopsis glycerol-3-phosphate permease 4 is localized in the plastids and involved in the accumulation of seed oil

被引：8

作者：

Kawai, Hiromitsu ^{[1
]}

Ishikawa, Toshiki ^{[1
]}

Mitsui, Toshiaki ^{[2
]}

Kore-eda, Shin ^{[3
]}

Kawai-Yamada, Maki ^{[1
]}

Ohnishi, Jun-ichi ^{[1
]}

机构：

[1] Saitama Univ, Grad Sch Sci & Engn, Saitama 3388570, Japan

[2] Niigata Univ, Grad Sch Sci & Technol, Niigata 9502181, Japan

[3] Saitama Univ, Comprehens Anal Ctr Sci, Saitama 3388570, Japan

来源：

PLANT BIOTECHNOLOGY | 2014年 / 31卷 / 02期

关键词：

Arabidopsis; glycerol; 3-phosphate; plastid; transporter; triacylglycerol; SN-GLYCEROL-3-PHOSPHATE DEHYDROGENASE; ESCHERICHIA-COLI; GENE FAMILY; PHOSPHATE; TRANSPORT; GLYCEROL; TRANSFORMATION; PURIFICATION; EXPRESSION; RESISTANCE;

D O I：

10.5511/plantbiotechnology.14.0222a

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In plant cells, glycerol 3-phosphate (G3P) is apparently able to permeate the plastid envelope, but no specific transporter has been characterized so far. The Arabidopsis five G3Pp proteins have been predicted as putative G3P permeases because of their high homologies with the prokaryotic G3P/phosphate antiporter GlpT. In the present study, G3Pp4 was characterized in detail utilizing reverse genetic approaches. Promoter analysis using GUS expression revealed that G3Pp4 was expressed strongly throughout the embryos during late developmental stages, and the seed lipid contents decreased in two g3pp4 knockout mutants. An enhanced yellow fluorescent protein-fused G3Pp4 was localized in the plastids, functioned physiologically in A. thaliana, and had G3P-transport activity in E. coli. These results suggest that Arabidopsis G3Pp4 is a plastid envelope-localized G3P transporter and involved in accumulation of storage lipids in late embryogenesis.

引用

页码：159 / U80

页数：9

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