Evaluation of different extraction methods from pomegranate whole fruit or peels and the antioxidant and antiproliferative activity of the polyphenolic fraction

被引：144
作者：
Masci, Alessandra [1 ]
Coccia, Andrea [2 ]
Lendaro, Eugenio [2 ]
Mosca, Luciana [3 ]
Paolicelli, Patrizia [4 ]
Cesa, Stefania [4 ]
机构：
[1] Univ Roma La Sapienza, Dipartimento Med Sperimentale, Unita Ric Sci Alimentaz & Nutr Umana, Viale Policlin 155, I-00161 Rome, Italy
[2] Univ Roma La Sapienza, Dipartimento Sci & Biotecnol Medchirurg, Corso Repubbl 79, I-04100 Latina, Italy
[3] Univ Roma La Sapienza, Dipartimento Sci Biochim, Piazzale Aldo Moro 5, I-00185 Rome, Italy
[4] Univ Roma La Sapienza, Dipartimento Chim & Tecnol Farmaco, Piazzale Aldo Moro 5, I-00185 Rome, Italy
来源：
FOOD CHEMISTRY | 2016年 / 202卷
关键词：
Pomegranate; Ellagic acid; HPLC-DAD; Antioxidant capacity; Antiproliferative activity; Human bladder cancer T24 cells; ELLAGIC ACID; CANCER; JUICE; ELLAGITANNINS; ANTHOCYANINS; PURIFICATION; PREVENTION; MIGRATION; CAPACITY; INVASION;
D O I：
10.1016/j.foodchem.2016.01.106
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Pomegranate is a functional food of great interest, due to its multiple beneficial effects on human health. This fruit is rich in anthocyanins and ellagitannins, which exert a protective role towards degenerative diseases. The aim of the present work was to optimize the extraction procedure, from different parts of the fruit, to obtain extracts enriched in selected polyphenols while retaining biological activity. Whole fruits or peels of pomegranate cultivars, with different geographic origin, were subjected to several extraction methods. The obtained extracts were analyzed for polyphenolic content, evaluated for antioxidant capacity and tested for antiproliferative activity on human bladder cancer T24 cells. Two different extraction procedures, employing ethyl acetate as a solvent, were useful in obtaining extracts enriched in ellagic acid and/or punicalagins. Antioxidative and antiproliferative assays demonstrated that the antioxidant capability is directly related to the phenolic content, whereas the antiproliferative activity is to be mainly attributed to ellagic acid. (C) 2016 Elsevier Ltd. All rights reserved.
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收藏
页码：59 / 69
页数：11
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