Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source

被引：21

作者：

Ren, Kangning ^{[1
]}

Liang, Qionglin ^{[1
]}

Mu, Xuan ^{[2
]}

Luo, Guoan ^{[1
,2
]}

Wang, Yiming ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ, Beijing 100084, Peoples R China

[2] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

来源：

LAB ON A CHIP | 2009年 / 9卷 / 05期

关键词：

FLUORESCENCE DETECTION; SAMPLE INTRODUCTION; HIGH-SPEED; MICROCHIP; DEVICE;

D O I：

10.1039/b810705j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel miniaturized, portable fluorescence detection system for capillary array electrophoresis (CAE) on a microfluidic chip was developed, consisting of a scanning light-emitting diode (LED) light source and a single point photoelectric sensor. Without charge coupled detector (CCD), lens, fibers and moving parts, the system was extremely simplified. Pulsed driving of the LED significantly increased the sensitivity, and greatly reduced the power consumption and photobleaching effect. The highly integrated system was robust and easy to use. All the advantages realized the concept of a portable micro-total analysis system (m-TAS), which could work on a single universal serial bus (USB) port. Compared with traditional CAE detecting systems, the current system could scan the radial capillary array with high scanning rate. An 8-channel CAE of fluorescein isothiocyanate (FITC) labeled arginine (Arg) on chip was demonstrated with this system, resulting in a limit of detection (LOD) of 640 amol.

引用

页码：733 / 736

页数：4

共 22 条

[1] Replica multichannel polymer chips with a network of sacrificial channels sealed by adhesive printing method [J].

Dang, F ;

Shinohara, S ;

Tabata, O ;

Yamaoka, Y ;

Kurokawa, M ;

Shinohara, Y ;

Ishikawa, M ;

Baba, Y .

LAB ON A CHIP, 2005, 5 (04) :472-478

[2] Micro total analysis systems. Latest advancements and trends [J].

Dittrich, Petra S. ;

Tachikawa, Kaoru ;

Manz, Andreas .

ANALYTICAL CHEMISTRY, 2006, 78 (12) :3887-3907

[3] Thin-film polymer light emitting diodes as integrated excitation sources for microscale capillary electrophoresis [J].

Edel, JB ;

Beard, NP ;

Hofmann, O ;

DeMello, JC ;

Bradley, DDC ;

deMello, AJ .

LAB ON A CHIP, 2004, 4 (02) :136-140

[4] Microfabricated 384-lane capillary array electrophoresis bioanalyzer for ultrahigh-throughput genetic analysis [J].

Emrich, CA ;

Tian, HJ ;

Medintz, IL ;

Mathies, RA .

ANALYTICAL CHEMISTRY, 2002, 74 (19) :5076-5083

[5] A high-throughput continuous sample introduction interface for microfluidic chip-based capillary electrophoresis systems [J].

Fang, Q ;

Xu, GM ;

Fang, ZL .

ANALYTICAL CHEMISTRY, 2002, 74 (06) :1223-1231

[6] An absorption detection approach for multiplexed capillary electrophoresis using a linear photodiode array [J].

Gong, XY ;

Yeung, ES .

ANALYTICAL CHEMISTRY, 1999, 71 (21) :4989-4996

[7] Tilted Brownian ratchet for DNA analysis [J].

Huang, LR ;

Cox, EC ;

Austin, RH ;

Sturm, JC .

ANALYTICAL CHEMISTRY, 2003, 75 (24) :6963-6967

[8] Acousto-optical deflection-based laser beam scanning for fluorescence detection on multichannel electrophoretic microchips [J].

Huang, Z ;

Munro, N ;

Huhmer, AFR ;

Landers, JP .

ANALYTICAL CHEMISTRY, 1999, 71 (23) :5309-5314

[9] CAPILLARY ARRAY ELECTROPHORESIS - AN APPROACH TO HIGH-SPEED, HIGH-THROUGHPUT DNA SEQUENCING [J].

MATHIES, RA ;

HUANG, XC .

NATURE, 1992, 359 (6391) :167-169

[10] Whole column fluorescence imaging on a microchip by using a programmed organic light emitting diode array as a spatial-scanning light source and a single photomultiplier tube as detector [J].

Ren, Kangning ;

Liang, Qionglin ;

Yao, Bo ;

Luo, Guoan ;

Wang, Liduo ;

Gao, Yudi ;

Wang, Yiming ;

Qiu, Yong .

LAB ON A CHIP, 2007, 7 (11) :1574-1580

← 1 2 3 →