Metabonomics Approach to Understanding Acute and Chronic Stress in Rat Models

被引：66

作者：

Wang, Xiaoyan ^{[1
,2
]}

Zhao, Tie ^{[1
,2
]}

Qiu, Yunping ^{[1
,2
]}

Su, Mingming ^{[1
,2
]}

Jiang, Tao ^{[3
]}

Zhou, Mingmei ^{[3
]}

Zhao, Aihua ^{[1
,2
]}

Jia, Wei ^{[4
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Shanghai 200240, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China

[3] Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China

[4] Univ N Carolina, Dept Nutr, Greensboro, NC 28081 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 05期

关键词：

metabonomics; acute stress; chronic stress; urine; gas chromatography/mass spectrometry; multivariate analysis; METABOLIC PROFILING REVEALS; CHRONIC VARIABLE STRESS; CHRONIC MILD STRESS; ACID SUPPLEMENTATION; COLD STRESS; DEPRESSION; RECEPTOR; TYROSINE; PLASMA; MUSCLE;

D O I：

10.1021/pr801086k

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of acute and chronic stress on the production of systemic metabolites were investigated in male Sprague-Dawley (SD) rats. Metabolites excreted in urine were analyzed using GUMS in conjunction with multivariate and univariate statistical techniques. SD rats were subjected to two kinds of acute stress and chronic unpredictable mild stress, respectively. Metabolic analysis demonstrated that urinary expression of a number of metabolites including glutamate, glutamine, homovanillate, proline, succinate, citrate, and tyrosine altered in the acute stress model in the same way as in the chronic model, while pimelate and hippurate changed in the opposite trend. The results suggested that the stress induced metabolic perturbations were reversible and nonspecific. Metabolic response to chronic combined stress revealed biochemical clues to depression-like symptoms validated by behavior and physiologic results. This study provides a noninvasive and dynamic analytical strategy for the characterization of endogenous metabolic perturbations induced by external stress.

引用

页码：2511 / 2518

页数：8

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