Release of netilmicin and vancomycin from cancellous bone

被引:40
作者
Witso, E [1 ]
Persen, L
Benum, P
Bergh, K
机构
[1] Norwegian Univ Sci & Technol, Dept Orthopaed Surg, NO-7006 Trondheim, Norway
[2] Norwegian Univ Sci & Technol, Univ Hosp, Dept Microbiol, NO-7006 Trondheim, Norway
[3] Norwegian Univ Sci & Technol, Dept Lab Med, NO-7006 Trondheim, Norway
来源
ACTA ORTHOPAEDICA SCANDINAVICA | 2002年 / 73卷 / 02期
关键词
D O I
10.1080/000164702753671812
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
First, we studied the effect of the following variables used for netilmicin- and vancomycin-impregnation of cancellous bone: a) antibiotic concentration of the impregnation fluid, b) time used for impregnation, c) pH of the impregnation fluid, d) the degree of bone morselizing and e) antibiotic combination. An increase in the antibiotic concentration of the impregnation fluid increased the amount of antibiotics released from bone. In addition, the amount of vancomycin eluted was also dependent on the time used for impregnation. The fraction of the total amount of netilmicin and vancomycin released after 24 h was 80% and 30%, respectively. More netilmicin and vancomycin were eluted from bone impregnated with antibiotics at pH 7 than the amount eluted from bone impregnated at pH 3. More netilmicin was eluted from fine morselized bone than from coarse morselized bone. By combining netilmicin and vancomycin in the impregnation fluid, the release of vancomycin was reduced. Secondly, we analyzed if the release of antibiotics from bone was complete: 99.9% of the total amount of netilmicin adsorbed to the bone was released by elution during 6 weeks. Finally, after implantation of netilmicin-impregnated bone in rabbit femur condyle, we measured netilmicin and vancomycin in serum: peak serum values of netilmicin were 4.2 (3.7-4.7) mg/L 2-3 h postoperatively.
引用
收藏
页码:199 / 205
页数:7
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