A conventional PCR technique to detect Septoria tritici in wheat seeds

被引：14

作者：

Consolo, V. F. ^{[1
]}

Albani, C. M. ^{[1
]}

Beron, C. M. ^{[1
]}

Salerno, G. L. ^{[1
]}

Cordo, C. A. ^{[2
]}

机构：

[1] CEBB CIB FIBA, RA-7600 Mar Del Plata, Argentina

[2] Univ Nacl La Plata, CIC, CIDEFI, Fac Ciencias Agr & Forestales, RA-1900 La Plata, Buenos Aires, Argentina

来源：

AUSTRALASIAN PLANT PATHOLOGY | 2009年 / 38卷 / 03期

关键词：

Mycosphaerella graminicola; seedborne pathogen; Septoria leaf blotch; wheat; CHAIN-REACTION ASSAYS; REAL-TIME PCR; MYCOSPHAERELLA-GRAMINICOLA; RHYNCHOSPORIUM-SECALIS; STAGONOSPORA-NODORUM; PRIMERS; BARLEY; DNA; DIFFERENTIATION; IDENTIFICATION;

D O I：

10.1071/AP08099

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A PCR assay was developed for detection of wheat seed naturally contaminated with Septoria tritici. S. tritici specific primers were derived from strict alignment of ITS and alpha-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from S. tritici isolates with all primer pairs, whereas no product was generated from other DNA sources. S. tritici was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of S. tritici genomic DNA was possible. This is the first report on the detection of S. tritici DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.

引用

页码：222 / 227

页数：6

共 41 条

[1] Diagnostics of phytopathogen fungi Septoria tritici and Stagonospora nodorum by fluorescent amplification-based specific hybridization (FLASH) PCR [J].