NIR-II pH Sensor with a FRET Adjustable Transition Point for In Situ Dynamic Tumor Microenvironment Visualization

被引：128

作者：

Zhao, Mengyao ^{[1
]}

Wang, Jianbo ^{[1
,2
]}

Lei, Zuhai ^{[1
]}

Lu, Lingfei ^{[1
]}

Wang, Shangfeng ^{[1
]}

Zhang, Hongxin ^{[1
]}

Li, Benhao ^{[1
]}

Zhang, Fan ^{[1
]}

机构：

[1] Fudan Univ, Dept Chem, State Key Lab Mol Engn Polymers & iChem, Shanghai Key Lab Mol Catalysis & Innovat Mat, Shanghai 200433, Peoples R China

[2] Jiaxing Univ, Coll Biol Chem Sci & Engn, Jiaxing 314001, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2021年 / 60卷 / 10期

基金：

中国国家自然科学基金; 国家重点研发计划; 中国博士后科学基金;

关键词：

dyes; pigments; fluorescence; imaging agents; near infrared; sensors; BROAD RANGE; CANCER;

D O I：

10.1002/anie.202012021

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Monitoring the pH in tumor lesions provides abundant physiological information. However, currently developed pH sensors only achieve sensitive detection in the settled response region around the pH transition point (pH(t)). To realize tumor pH monitoring with high sensitivity within a wider response region, reported here are serial pH(t) adjustable sensors (pTAS) that simply regulate the component ratio of second near-infrared (NIR-II) emission aza-BODIPY (NAB) donor and pH sensitive rhodamine-based pre-acceptor (NRh) in Forster resonance energy transfer system. Combining the pH response regions of pTAS, a twofold widened pH detection range (6.11-7.22) is obtained compared to the pH(t) settled sensor (6.38-6.94). With an adjustable pH(t), in vivo tumor pH increase and decrease processes could be dynamically visualized through dual-channel ratiometric bioimaging within the NIR-II window, with a coefficient of variation under 1 % compared to the standard pH meter.

引用

页码：5091 / 5095

页数：5

共 36 条

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