Role of heme oxygenase-1 in human placenta on iron supply to fetus

被引:8
作者
Inoue, Rikako [1 ]
Irie, Yasuyuki [1 ]
Akagi, Reiko [2 ]
机构
[1] Okayama Prefectural Univ, Fac Hlth & Welf Sci, Dept Nutr Sci, 111 Kuboki, Soja, Okayama 7191197, Japan
[2] Yasuda Womens Univ, Fac Pharm, Dept Pharm, 6-13-1 Yasuhigashi, Hiroshima 7310153, Japan
关键词
Heme oxygenase-1; Transferrin receptor-1; Ferroportin; 1; Iron deposit; Placenta; Villous tissue; DEVELOPMENTAL-CHANGES; FETAL-GROWTH; EXPRESSION; FERRITIN; HO-2; BED;
D O I
10.1016/j.placenta.2020.09.065
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Introduction: To date, details on how iron is supplied from the mother to the fetus through the placenta have remained unclear. Recently, increasing evidence has shown that heme oxygenase (HO)-1, which is an inducible isoform of the rate-limiting enzyme in the heme degradation pathway, may be involved in the effective reutilization of iron. In this study, we examined the distribution and gene expression of HO-1 in the villous tissue of human placenta at various periods of pregnancy. Methods: Using the placenta of 38 samples for which consent was obtained, chronological changes in the localization of HO-1 protein were examined by histological examination. RT-PCR was also performed to examine the expression of HO-1, transferrin receptor-1, and ferroportin 1. Ferric iron in the tissues was analyzed by Prussian blue staining. Results: Immunohistochemical studies showed that HO-1 protein was exclusively expressed in trophoblastic cells throughout gestation. In the miscarriage placenta in the first trimester, ho-1 mRNA levels were significantly higher than normal. Placenta with fetal death (miscarriage) in the first and second trimester indicate significantly higher ratio of ho-1 gene for iron production to the fpn-1 gene for iron excretion than normal. These suggest that the role of HO-1 with various physiological functions is changing throughout pregnancy. Discussion: These findings suggest that HO-1 in placenta plays an important role in iron supplying system in the second trimester to support fetal development.
引用
收藏
页码:53 / 58
页数:6
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