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CTX-M-Type Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolated from Cases of Bovine Mastitis in Japan
被引:25
作者:
Saishu, Nobukazu
[1
,2
]
Ozaki, Hiroichi
[2
,3
]
Murase, Toshiyuki
[2
,3
]
机构:
[1] Tottori Prefectural Kurayoshi Livestock Hyg Serv, Kurayoshi, Tottori 6820017, Japan
[2] Yamaguchi Univ, United Grad Sch Vet Med, Yamaguchi 7538515, Japan
[3] Tottori Univ, Lab Vet Microbiol, Fac Agr, Tottori 6808553, Japan
关键词:
bovine mastitis;
ESBL;
Klebsiella pneumonia;
FOOD-PRODUCING ANIMALS;
ESCHERICHIA-COLI;
RAW-MILK;
PLASMIDS;
FARM;
ENTEROBACTERIACEAE;
CATTLE;
D O I:
10.1292/jvms.13-0120
中图分类号:
S85 [动物医学(兽医学)];
学科分类号:
0906 ;
摘要:
Three Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamase (ESBL) were obtained from three dairy cows with clinical mastitis in two farms in western Japan. Two of the 3 isolates from cows in different farms were able to transfer plasmids carrying the bla(CTX-M-2) gene to Escherichia coli recipient. Pulsed-field gel electrophoresis (PFGE) patterns of the 2 isolates were different from each other, although restricted-fragment patterns of the two conjugative plasmids were similar to each other. Additionally, PCR-based replicon typing revealed that both the plasmids belonged to type Inc.T. These results suggest that ESBL-encoding genes can be distributed in bacteria on dairy farms through the plasmids.
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页码:1153 / 1156
页数:4
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