Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales, Phaeophyta)

被引：26
作者：
Jiang, P
Qin, S [1 ]
Tseng, CK
机构：
[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2002年 / 47卷 / 17期
基金：
中国国家自然科学基金;
关键词：
Laminaria japonica; genetic engineering; hepatitis B surface antigen (HBsAg); expression system; vaccine;
D O I：
10.1360/02tb9317
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A transformation model for Laminaria japonica was established from 1993 to 1998, on the basis of which the transgenic kelp with heterologous gene encoding hepatitis B surface antigen (HBsAg) was obtained by using the micro-particle bombardment transformation method. Results of quantitative ELISA showed that HBsAg in transgenic kelp was 0.529 mug/mg soluble proteins on average and the highest value was 2.497 mug/mg, implying that recombinant HBsAg had natural epitope. Further support for the integration of HBsAg gene into kelp genome was obtained by PCR-Southern and total DNA hybridization. Prospect of kelp bio-reactor producing high value materials such as edible HBV vaccine was discussed as well.
引用
收藏
页码：1438 / 1440
页数：3
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