Chronic treatment with Sildenafil has no effect on folliculogenesis or fertility in C57BL/6 and C57BL/6 knockout for iNOS mice

被引：5
|
作者：
Matos Donato, Mariana Aragao [1 ]
Ribeiro, Edlene Lima [1 ]
Cipriano Torres, Dilenia de Oliveira [1 ]
Soares e Silva, Amanda Karolina [1 ]
dos Santos Gomes, Fabiana Oliveira [1 ]
e Silva, Bruna Santos [1 ]
Santos Rocha, Sura Wanessa [1 ]
Peixoto, Christina Alves [1 ,2 ]
机构：
[1] Fiocruz MS, Inst Aggeu Magalhaes, Lab Ultraestrutura, BR-50670420 Recife, PE, Brazil
[2] Ctr Tecnol Estrateg Nordeste, Lab Microscopia & Microanal, BR-50740540 Recife, PE, Brazil
来源：
TISSUE & CELL | 2015年 / 47卷 / 05期
关键词：
Ovary; Sildenafil; PDE5; NITRIC-OXIDE SYNTHASE; MEIOTIC MATURATION; CYCLIC-GMP; PHOSPHODIESTERASE-5; INHIBITOR; PULMONARY-HYPERTENSION; ERECTILE DYSFUNCTION; FOLLICLE DEVELOPMENT; GUANYLATE-CYCLASE; CORPUS-LUTEUM; MOUSE OOCYTES;
D O I：
10.1016/j.tice.2015.07.001
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
Sildenafil is an important phosphodiesterase inhibitor used to treat a range of diseases, including cardiovascular disease, prostatic hyperplasia and pulmonary hypertension. Its main mechanism of action is the inhibition of phosphodiesterase 5, leading to increased intracellular cyclic guanosine monophosphate. This second messenger plays an interesting role in the reproductive tract. The aim of the present study was to evaluate the effect of Sildenafil on folliculogenesis and fertility in mice. To do so, C57BL/6 wild-type mice and inducible nitric oxide synthase knockout (iNOS(-/-)) mice were treated with Sildenafil, and reproductive variables were evaluated. The treated and control animals underwent estrous cycle and fertility assay. Lipid profile, serum nitric oxide levels and the expression of endothelial nitric oxide synthase, inducible nitric oxide synthase and guanylate cyclase were evaluated. Additionally, ovaries were submitted to histological and morphological analysis. The findings demonstrated that chronic treatment with Sildenafil had no effect on folliculogenesis or fertility in C57BL/6 mice, suggesting that this drug can be safely used by women of childbearing age. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：515 / 525
页数：11
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