Roseisolibacter agri gen. nov., sp nov., a novel slow-growing member of the under-represented phylum Gemmatimonadetes

被引:31
作者
Pascual, Javier [1 ]
Foesel, Baerbel U. [1 ]
Geppert, Alicia [1 ]
Huber, Katharina J. [1 ]
Boedeker, Christian [1 ]
Luckner, Manja [2 ]
Wanner, Gerhard [2 ]
Overmann, Joerg [1 ,3 ]
机构
[1] Leibniz Inst DSMZ Deutsch Sammlung Mikroorganisme, Dept Microbial Ecol & Divers Res, Braunschweig, Germany
[2] Ludwig Maximilian Univ Munich, Dept Biol 1, Biozentrum, Planegg Martinsried, Germany
[3] Tech Univ Carolo Wilhelmina Braunschweig, Braunschweig, Germany
关键词
Gemmatimonadetes; Gemmatimonadaceae; savannah soil; 16S RIBOSOMAL-RNA; PERFORMANCE LIQUID-CHROMATOGRAPHY; SEMIARID SAVANNA SOIL; SUBDIVISION; 4; BACTERIA; AURANTIACA; SEQUENCES; DATABASE;
D O I
10.1099/ijsem.0.002619
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel slow-growing bacterium, designated strain AW1220(T), was isolated from agricultural floodplain soil sampled at Mashare (Kavango region, Namibia) by using a high-throughput cultivation approach. Strain AW1220(T) was characterized as a Gram-negative, non-motile, rod-shaped bacterium. Occasionally, some cells attained an unusual length of up to 35 mu m. The strain showed positive responses for catalase and cytochrome-c oxidase and divided by binary fission and/or budding. The strain had an aerobic chemoorganoheterotrophic metabolism and was also able to grow under micro-oxic conditions. Colonies were small and pink pigmented. Strain AW1220(T) was found to be a mesophilic, neutrophilic and non-halophilic bacterium. Cells accumulated polyphosphate intracellularly and mainly utilized complex protein substrates for growth. 16S rRNA gene sequence comparisons revealed that strain AW1220(T) belonged to the class Gemmatimonadetes (= group 1). Its closest relatives were found to be Gemmatimonas aurantiaca T-27(T) (90.9% gene sequence similarity), Gemmatimonas phototrophica AP64(T) (90.8 %) and Longimicrobium terrae CB-286315(T) (84.2 %). The genomic G+C content was 73.3 mol%. The major fatty acids were iso-C-15 : 0, C-16 : 1 omega 7c and/or iso-C-15 : 0 2-OH, iso-C-17 : 1 omega 9c, iso-C-15 : 0 3-OH and C-16 : 0. The predominant respiratory quinone was MK-9, albeit minor amounts of MK-8 and MK-10 are also present. The polar lipids comprised major amounts of phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol and one unidentified phosphoglycolipid. On the basis of its polyphasic characterization, strain AW1220(T) represents a novel genus and species of the class Gemmatimonadetes for which the name Roseisolibacter agri gen. nov., sp. nov. is proposed, with the type strain AW1220(T) (= DSM 104292(T) = LMG 29977(T)).
引用
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页码:1028 / 1036
页数:9
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