The purification and characterization of a novel lipid transfer protein from caryopsis of barnyard grass (Echinochloa crusgalli)

被引：10
|
作者：
Rogozhin, E. A. [1 ]
Odintsova, T. I. [2 ]
Musolyamov, A. Kh. [1 ]
Smirnov, A. N. [3 ]
Babakov, A. V. [4 ]
Egorov, Ts. A. [1 ]
Grishin, E. V. [1 ]
机构：
[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
[2] Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 117809, Russia
[3] Russian State Agr Univ, Timiryazev Moscow Agr Acad, Moscow 127550, Russia
[4] Russian State Agr Sci, Inst Agr Biotechnol, Moscow 127550, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2009年 / 45卷 / 04期
基金：
俄罗斯基础研究基金会;
关键词：
ANTIFUNGAL ACTIVITY; MULTIGENE FAMILY; BINDING PROTEINS; EXPRESSION; BARLEY; BACTERIAL; PEPTIDES;
D O I：
10.1134/S0003683809040024
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel lipid transfer protein called Ec-LTP was isolated from resting caryopsis of weed barnyard grass Echinochloa crusgalli (L.) Beauv.; its molecular weight, amino acid content and N-terminal amino acid sequence were determined. Ec-LTP was a 9150 D protein, containing eight cysteine residues, which formed four disulfide bonds. The isolated protein could significantly inhibit the development of pathogenic fungi Phytophthora infestans and Helminthosporium sativum, causing the late blight of potato and tomato and the root rot of herbs, respectively.
引用
收藏
页码：363 / 368
页数：6
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