A rapid method for estimation of the efficacy of potential antimicrobials in humans and animals by agar diffusion assay

被引：8
作者：
Salina, Elena G. [1 ]
Ekins, Sean [2 ]
Makarov, Vadim A. [1 ]
机构：
[1] Russian Acad Sci, Res Ctr Biotechnol, Bach Inst Biochem, Moscow, Russia
[2] Collaborat Pharmaceut Inc, Raleigh, NC USA
来源：
CHEMICAL BIOLOGY & DRUG DESIGN | 2019年 / 93卷 / 06期
基金：
俄罗斯基础研究基金会;
关键词：
agar diffusion assay; antimicrobial activity; clinical trials; drug candidate; macozinone; Tuberculosis; MICROBIOLOGICAL ANTIBIOTIC ASSAY; DISC PLATE METHOD; MYCOBACTERIUM-TUBERCULOSIS; DISCOVERY; SMEGMATIS; DRUGS; THERAPIES; REGIMENS; MODEL;
D O I：
10.1111/cbdd.13427
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Drug resistance continues to challenge traditional antimicrobial drugs and limit their clinical utility. This requires us to continue our search for new drug candidates with novel mechanisms of action against infectious diseases. We now describe a simple agar diffusion assay, which can be used as a general method for the rapid detection of antimicrobial activity of drug candidates in animal or human blood plasma for the ultimate prediction of the efficacy of potential drugs prior to clinical trials. We present an example for a clinical candidate against Mycobacterium tuberculosis.
引用
收藏
页码：1021 / 1025
页数：5
相关论文
共 20 条
  • [1] Evaluation of the Mycobacterium smegmatis and BCG models for the discovery of Mycobacterium tuberculosis inhibitors
    Altaf, Mudassar
    Miller, Christopher H.
    Bellows, David S.
    O'Toole, Ronan
    [J]. TUBERCULOSIS, 2010, 90 (06) : 333 - 337
  • [2] New small-molecule synthetic antimycobacterials
    Ballell, L
    Field, RA
    Duncan, K
    Young, RJ
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2005, 49 (06) : 2153 - 2163
  • [3] DISC PLATE METHOD OF MICROBIOLOGICAL ANTIBIOTIC ASSAY .1. FACTORS INFLUENCING VARIABILITY AND ERROR
    DAVIS, WW
    STOUT, TR
    [J]. APPLIED MICROBIOLOGY, 1971, 22 (04) : 659 - &
  • [4] DISC PLATE METHOD OF MICROBIOLOGICAL ANTIBIOTIC ASSAY .2. NOVEL PROCEDURE OFFERING IMPROVED ACCURACY
    DAVIS, WW
    STOUT, TR
    [J]. APPLIED MICROBIOLOGY, 1971, 22 (04) : 666 - &
  • [5] Machine Learning Model Analysis and Data Visualization with Small Molecules Tested in a Mouse Model of Mycobacterium tuberculosis Infection (2014-2015)
    Ekins, Sean
    Perryman, Alexander L.
    Clark, Alex M.
    Reynolds, Robert C.
    Freundlich, Joel S.
    [J]. JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2016, 56 (07) : 1332 - 1343
  • [6] Fauci AS, 2012, NEW ENGL J MED, V366, P454, DOI [10.1056/NEJMc1202013, 10.1056/NEJMc1204960]
  • [7] DISC-AGAR DIFFUSION MICRIBIOLOGICAL ASSAY PROCEDURE FOR DETERMINING SERUM AND URINE LEVELS OF SULFACYTINE AND OTHER SULFONAMIDES
    HEIFETZ, CL
    CHODUBSKI, JA
    DECARLO, MO
    FISHER, MW
    [J]. APPLIED MICROBIOLOGY, 1971, 21 (05) : 893 - +
  • [8] Towards a new combination therapy for tuberculosis with next generation benzothiazinones
    Makarov, Vadim
    Lechartier, Benoit
    Zhang, Ming
    Neres, Joao
    van der Sar, Astrid M.
    Raadsen, Susanne A.
    Hartkoorn, Ruben C.
    Ryabova, Olga B.
    Vocat, Anthony
    Decosterd, Laurent A.
    Widmer, Nicolas
    Buclin, Thierry
    Bitter, Wilbert
    Andries, Koen
    Pojer, Florence
    Dyson, Paul J.
    Cole, Stewart T.
    [J]. EMBO MOLECULAR MEDICINE, 2014, 6 (03) : 372 - 383
  • [9] Benzothiazinones Kill Mycobacterium tuberculosis by Blocking Arabinan Synthesis
    Makarov, Vadim
    Manina, Giulia
    Mikusova, Katarina
    Moellmann, Ute
    Ryabova, Olga
    Saint-Joanis, Brigitte
    Dhar, Neeraj
    Pasca, Maria Rosalia
    Buroni, Silvia
    Lucarelli, Anna Paola
    Milano, Anna
    De Rossi, Edda
    Belanova, Martina
    Bobovska, Adela
    Dianiskova, Petronela
    Kordulakova, Jana
    Sala, Claudia
    Fullam, Elizabeth
    Schneider, Patricia
    McKinney, John D.
    Brodin, Priscille
    Christophe, Thierry
    Waddell, Simon
    Butcher, Philip
    Albrethsen, Jakob
    Rosenkrands, Ida
    Brosch, Roland
    Nandi, Vrinda
    Bharath, Sowmya
    Gaonkar, Sheshagiri
    Shandil, Radha K.
    Balasubramanian, Venkataraman
    Balganesh, Tanjore
    Tyagi, Sandeep
    Grosset, Jacques
    Riccardi, Giovanna
    Cole, Stewart T.
    [J]. SCIENCE, 2009, 324 (5928) : 801 - 804
  • [10] Learning from the past for TB drug discovery in the future
    Mikusova, Katarina
    Ekins, Sean
    [J]. DRUG DISCOVERY TODAY, 2017, 22 (03) : 534 - 545
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