NMR resonance assignment of DnaE intein from Nostoc punctiforme

被引：8

作者：

Heinamaki, Kimmo ^{[1
]}

Oeemig, Jesper S. ^{[1
]}

Djupsjobacka, Janica ^{[1
]}

Iwai, Hideo ^{[1
]}

机构：

[1] Univ Helsinki, Inst Biotechnol, Res Program Struct Biol & Biophys, Helsinki 00014, Finland

来源：

BIOMOLECULAR NMR ASSIGNMENTS | 2009年 / 3卷 / 01期

基金：

芬兰科学院;

关键词：

Protein splicing; Intein; Protein trans-splicing; IN-VIVO; PROTEIN;

D O I：

10.1007/s12104-008-9137-1

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

DnaE intein from Nostoc punctiforme (Npu) is one of naturally occurring split inteins, which has robust protein splicing activity. Highly efficient trans-splicing activity of NpuDnaE intein could widen various biotechnological applications. However, structural basis of the efficient protein splicing activity is poorly understood. As a first step toward better understanding of protein trans-splicing mechanism, we present the backbone and side-chain resonance assignments of a single chain variant NpuDnaE intein as determined by triple resonance experiments with [C-13,N-15]-labeled protein.

引用

页码：41 / 43

页数：3

共 11 条

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