Modification of carboxymethyl cellulose grafted with collagen peptide and its antioxidant activity

被引：48

作者：

Fan, Lihong ^{[1
]}

Peng, Min ^{[1
]}

Zhou, Xiaoyu ^{[1
]}

Wu, Huan ^{[1
]}

Hu, Jin ^{[1
]}

Xie, Weiguo ^{[2
]}

Liu, Shuhua ^{[2
]}

机构：

[1] Wuhan Univ Technol, Coll Chem Engn, Wuhan 430070, Peoples R China

[2] Third Hosp Wuhan, Wuhan 430060, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2014年 / 112卷

基金：

中国国家自然科学基金;

关键词：

Carboxymethyl cellulose; Collagen peptide; Antioxidant activity; Free radical; Fibroblasts cells; OXYGEN-FREE-RADICALS; MOLECULAR-WEIGHT; POLYETHYLENE; COMPOSITE; PROTEASE;

D O I：

10.1016/j.carbpol.2014.05.056

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Carboxymethyl cellulose used in wounds has little antioxidant capacity. The aim of the present study was to improve the scavenging ability of carboxymethyl cellulose by modified with collagen peptide. The reaction conditions have been optimized by varying mass ratio of collagen peptide to carboxymethyl cellulose, temperature and reaction time. Antioxidant activities of carboxymethyl cellulose derivatives (CMCC) were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide radicals and the reducing power. The effects of concentration, degree of substitution (DS) and molecular weight on three different radicals scavenging activity and reducing power were examined. Methylthiazol tetrazolium (MTT) assay was used to evaluate the fibroblasts cells cytotoxicity of CMCC. Results showed that the scavenging effects of CMCC increased with the increasing of DS and concentration. This product of CMCC possesses a distinct antioxidant capacity on radicals. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：32 / 38

页数：7

共 38 条

[1] Polyvinyl alcohol-polyethylene oxide-carboxymethyl cellulose membranes for drug delivery [J].

Agarwal, Roopali ;

Alam, M. Sarwar ;

Gupta, Bhuvanesh .

JOURNAL OF APPLIED POLYMER SCIENCE, 2013, 129 (06) :3728-3736

[2] Water sorption behaviour of highly swelling (carboxy methylcellulose-g-polyacrylamide) hydrogels and release of potassium nitrate as agrochemical [J].

Bajpai, AK ;

Giri, A .

CARBOHYDRATE POLYMERS, 2003, 53 (03) :271-279

[3] Collagen-grafted ultra-high molecular weight polyethylene for biomedical applications [J].

Bockova, Jindriska ;

Vojtova, Lucy ;

Prikryl, Radek ;

Cechal, Jan ;

Jancar, Josef .

CHEMICAL PAPERS, 2008, 62 (06) :580-588

[4] Chitosan/carboxymethyl cellulose polyelectrolyte complex scaffolds for pulp cells regeneration [J].

Chen, Huangqin ;

Fan, Mingwen .

JOURNAL OF BIOACTIVE AND COMPATIBLE POLYMERS, 2007, 22 (05) :475-491

[5] Mechanism of action of PROMOGRAN, a protease modulating matrix, for the treatment of diabetic foot ulcers [J].

Cullen, B ;

Smith, R ;

McCulloch, E ;

Silcock, D ;

Morrison, L .

WOUND REPAIR AND REGENERATION, 2002, 10 (01) :16-25

[6] Preparation and characterization of sodium alginate modified with collagen peptides [J].

Fan, Lihong ;

Cao, Mi ;

Gao, Song ;

Wang, Tan ;

Wu, Huan ;

Peng, Min ;

Zhou, Xiaoyu ;

Nie, Min .

CARBOHYDRATE POLYMERS, 2013, 93 (02) :380-385

[7] Expression and Localization of Carnitine/Organic Cation Transporter OCTN1 and OCTN2 in Ocular Epithelium [J].

Garrett, Qian ;

Xu, Shunjiang ;

Simmons, Peter A. ;

Vehige, Joseph ;

Flanagan, Judith L. ;

Willcox, Mark D. .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2008, 49 (11) :4844-4849

[8] Hydrolyzed collagen improves bone metabolism and biomechanical parameters in ovariectomized mice: An in vitro and in vivo study [J].

Guillerminet, Fanny ;

Beaupied, Helene ;

Fabien-Soule, Veronique ;

Tome, Daniel ;

Benhamou, Claude-Laurent ;

Roux, Christian ;

Blais, Anne .

BONE, 2010, 46 (03) :827-834

[9]

Heinze T, 1999, ANGEW MAKROMOL CHEM, V266, P37

[10] Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis [J].

Je, Jae-Young ;

Qian, Zhong-Ji ;

Byun, Hee-Guk ;

Kim, Se-Kwon .

PROCESS BIOCHEMISTRY, 2007, 42 (05) :840-846

← 1 2 3 4 →