Alteration of HDL functionality and PON1 activities in acute coronary syndrome patients

被引:30
作者
Bounafaa, Abdelghani [1 ,2 ,4 ,5 ]
Berrougui, Hicham [2 ,5 ]
Ikhlef, Souade [5 ]
Essamadi, Abdelkhalid [1 ]
Nasser, Boubker [1 ]
Bennis, Ahmed [3 ]
Yamoul, Najoua [3 ]
Ghalim, Noreddine [4 ]
Khalil, Abdelouahed [5 ]
机构
[1] Hassan I Univ, Fac Sci & Technol, Appl Biochem & Toxicol Team, Lab Biochem & Neurosci, Settat, Morocco
[2] Sultan Moulay Sliman Univ, Polydisciplinary Fac, Dept Biol, Beni Mellal, Morocco
[3] Ibn Rochd Univ, Ctr Hosp, Serv Cardiol, Casablanca, Morocco
[4] Pasteur Inst Morocco, Biochem Lab, Casablanca, Morocco
[5] Univ Sherbrooke, Fac Med & Biol Sci, Dept Med, Geriatr Serv, Sherbrooke, PQ J1K 2R1, Canada
基金
加拿大健康研究院;
关键词
ACS; HDL; Cholesterol efflux; Oxidative stress; PON1; HIGH-DENSITY-LIPOPROTEIN; REVERSE CHOLESTEROL TRANSPORT; APOLIPOPROTEIN-A-I; PARAOXONASE ACTIVITY; EFFLUX; BINDING; RISK; CLOPIDOGREL; OXIDATION; MALONDIALDEHYDE;
D O I
10.1016/j.clinbiochem.2014.09.001
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objective: The functionality of HDL has been suggested as an important factor in the prevention of cardiovascular and coronary artery diseases. The objective of the present study was to investigate the functionality of HDL and the factors that may affect the anti-atherogenic properties of HDL in ACS patients. Methods and results: One hundred healthy subjects and 205 ACS patients were recruited. HDL functionality was evaluated by measuring their capacity to mediate cholesterol efflux from J774 macrophages. Oxidative stress status was determined by measuring plasma malondialdehyde (MDA), protein carbonyl, and vitamin E levels by HPLC. The PON1 Q192R polymorphism status and PON1 paraoxonase and arylesterase activities of the healthy subjects and ACS patients were also determined. The HDL of ACS patients displayed a limited capacity to mediate cholesterol efflux, especially via the ABCA1-pathway. MDA (7.06 +/- 0.29 mu M) and protein carbonyl (9.29 +/- 0.26 mu M) levels were significantly higher in ACS patients than in healthy subjects (2.29 +/- 0.21 mu M and 3.07 +/- 0.17 mu M, respectively, p < 0.0001), while alpha- and gamma-tocopherol (vitamin E) levels in ACS patients were 8-fold (p < 0.001) and 2-fold (p < 0.05) lower than in healthy subjects. Paraoxonase, arylesterase and HDL-corrected PON1 activities (PON1 activity/HDL ratio) were significantly lower in ACS patients. Logistic regression analyses showed that high PON1 paraoxonase and arylesterase activities had a significant protective effect (OR = 0.413, CI 0.289-0.590, p < 0.001; OR = 0.232 CI 0.107-0.499, p < 0.001, respectively) even when adjusted for HDL level, age, BMI, and PON1 polymorphism. Conclusion: The results of the present study showed that the functionality of HDL is impaired in ACS patients and that the impairment may be due to oxidative stress and an alteration of PON1 activities. (C) 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:318 / 325
页数:8
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