Polymerase chain reaction-based assays for the diagnosis of human brucellosis

被引：44

作者：

Wang, Ying ^{[1
]}

Wang, Zhanli ^{[1
]}

Zhang, Yaxian ^{[1
]}

Bai, Liyun ^{[1
]}

Zhao, Yue ^{[1
]}

Liu, Chunfang ^{[1
]}

Ma, An ^{[2
]}

Yu, Hui ^{[1
]}

机构：

[1] Baotou Med Coll, Baotou 014010, Peoples R China

[2] Zhejiang Acad Med Sci, Hangzhou 310013, Zhejiang, Peoples R China

来源：

ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS | 2014年 / 13卷

基金：

中国国家自然科学基金;

关键词：

Polymerase chain reaction techniques; Human brucellosis; Brucella spp; Molecular diagnosis; REAL-TIME PCR; FRAGMENT LENGTH POLYMORPHISM; PARAFFIN-EMBEDDED TISSUES; RAPID DIAGNOSIS; MULTIPLEX PCR; NESTED PCR; LABORATORY DIAGNOSIS; SERUM SAMPLES; WHOLE-BLOOD; SINGLE-TUBE;

D O I：

10.1186/s12941-014-0031-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Polymerase chain reaction (PCR) is an in vitro technique for the nucleic acid amplification, which is commonly used to diagnose infectious diseases. The use of PCR for pathogens detection, genotyping and quantification has some advantages, such as high sensitivity, high specificity, reproducibility and technical ease. Brucellosis is a common zoonosis caused by Brucella spp., which still remains as a major health problem in many developing countries around the world. The direct culture and immunohistochemistry can be used for detecting infection with Brucella spp. However, PCR has the potential to address limitations of these methods. PCR are now one of the most useful assays for the diagnosis in human brucellosis. The aim of this review was to summarize the main PCR techniques and their applications for diagnosis and follow-up of patients with brucellosis. Moreover, advantages or limitation of the different PCR methods as well as the evaluation of PCR results for treatment and follow-up of human brucellosis were also discussed.

引用

页数：8

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